miR-25 modulates triacylglycerol and lipid accumulation in goat mammary epithelial cells by repressing PGC-1beta.
miR-25 modulates triacylglycerol and lipid accumulation in goat mammary epithelial cells by repressing PGC-1beta.
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miR-25通过抑制PGC-1β调节山羊乳腺上皮细胞中的三酰甘油和脂质积累
DOI：
10.1186/s40104-018-0262-0
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发表时间：
2018
影响因子：
7
通讯作者：
Gou D
中科院分区：
文献类型：
--
作者：
Ma L;Qiu H;Chen Z;Li L;Zeng Y;Luo J;Gou D
山羊（Caprahircus）是最重要的家畜之一。羊奶脂肪是羊奶营养品质的重要组成部分。越来越多的证据表明microRNA（miRNAs）在脂质代谢中的关键作用。利用高灵敏度的S-poly（T）plus检测miRNAs的方法，分析了715个miRNAs在不同泌乳期山羊乳腺组织中的表达模式。我们观察到miR-25表达与产奶量呈负相关。miR-25的过表达显著抑制三酰甘油合成和脂滴积累。为探讨miR-25在乳脂代谢中的调控机制，采用生物信息学分析和3′-UTR分析相结合的方法，对miR-25的靶基因进行分析。过氧化物酶体增殖激活受体γ辅激活因子1 β（PGC-1 β）是脂肪生成的关键调节因子，被认为是miR-25的直接靶点，其3′-UTR内有3个特异性位点。此外，山羊乳腺上皮细胞中的miR-25模拟物降低了参与脂质代谢的基因的表达。综上所述，我们的研究结果表明miR-25可能参与脂质代谢，我们揭示了miR-25/PGC-1 β调节轴在哺乳期的功能。
The goat (Caprahircus) is one of the most important livestock animals. Goat milk fat is an important component in the nutritional quality of goat milk. Growing evidence points to the critical roles of microRNAs (miRNAs) in lipid metabolism. Using a highly sensitive method of S-poly(T) plus for miRNAs detection, we analyze the expression patterns of 715 miRNAs in goat mammary gland tissues at different stages of lactation. We observed that miR-25 expression had an inverse relationship with milk production. Overexpression of miR-25 significantly repressed triacylglycerol synthesis and lipid droplet accumulation. To explore the regulatory mechanism of miR-25 in milk lipid metabolism, we analyzed its putative target genes with bioinformatics analysis followed by 3′-UTR assays. Peroxisome proliferative activated receptor gamma coactivator 1 beta (PGC-1beta), a key regulator of lipogenics was identified as a direct target of miR-25 with three specific sites within its 3′-UTR. In addition, miR-25 mimics in goat mammary epithelial cells reduced the expressions of genes involved in lipid metabolism. Taken together, our results show miR-25 is potentially involved in lipid metabolism and we reveal the function of the miR-25/PGC-1beta regulatory axis during lactation.