Feasibility of caffeic acid as a crosslinking agent in modifying acellular extracellular matrices.
Feasibility of caffeic acid as a crosslinking agent in modifying acellular extracellular matrices.
复制标题
咖啡酸作为交联剂修饰无细胞细胞外基质的可行性。
DOI：
10.1016/j.bbrc.2023.08.024
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发表时间：
2023
影响因子：
3.1
通讯作者：
Min Wang
中科院分区：
文献类型：
--
作者：
Hongjie Ji;Haiming Zhang;Yuzhuo Wang;Zuhua Qiu;Jing;Jie Cao;Kexin Xu;Yi Zhang;Yanlin Jiang;Min Wang
Acellular extracellular matrices (aECM) are commonly utilized, both experimentally and clinically, in the regenerative medicine field. However, some disadvantages such as rapid degradation, poor mechanical properties, chronic inflammatory reactions and low antioxidant activity have limited their further application. In this study the feasibility of caffeic acid as a crosslinking agent in fixing small intestinal submucosa (SIS) was evaluated. The ninhydrin assay, swelling ratio and FTIR spectra indicated that caffeic acid can efficiently react with free amino groups to crosslink SIS and the highest crosslinking index reached 21.60 ± 1.37%. Moreover, the shrinkage temperature of SIS remarkably increased from 59 °C to about 80 °C and the degradation rate of CA-SIS was all lower than 6%, demonstrating their improved biostability and hydrothermal stability. Importantly, the antioxidant activity of CA-SIS ranged from 55% to 90%, statistically higher than that of native SIS (37.33 ± 2.94%). Additionally the cytotoxicity test presented that the cytotoxicity grade of CA-SIS was 1 or 0, whilst large numbers of living HUVECs were attached to the surface of the material and exhibited high cell viability. These results indicated their excellent cytocompatibility. The data of subcutaneous implant displayed that the number of inflammatory cells in 2%- and 2.5%CA-SIS groups remained at a low level (below 100 cells/field) while that of the native SIS group continued increasing, finally reaching 142.33 ± 30.92 cells/field. In conclusion, caffeic acid is a promising candidate for modifying aECM and may play a vital role in the design and fabrication of tissue engineering scaffolds.
DOI：
10.2106/jbjs.e.00742
发表时间：
2007-03
期刊：
The Journal of bone and joint surgery. American volume
影响因子：
--
作者：
T. Gilbert;A. M. Stewart-Akers;A. Simmons-Byrd;S. Badylak
通讯作者：
T. Gilbert;A. M. Stewart-Akers;A. Simmons-Byrd;S. Badylak