Stable and dynamic nucleosome states during a meiotic developmental process.
Stable and dynamic nucleosome states during a meiotic developmental process.
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DOI：
10.1101/gr.117465.110
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发表时间：
2011-06
期刊：
影响因子：
7
通讯作者：
Liye Zhang;Hong Ma;Frank Pugh
中科院分区：
文献类型：
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作者：
Liye Zhang;Hong Ma;Frank Pugh
染色体组织的可塑性是未知的，因为染色体经历了一系列完整的过程，包括DNA复制、重组、染色质致密化和发育过程中转录的变化。我们生成了酵母减数分裂孢子发育（配子发生）过程中单个核小体组织状态的全基因组图谱，包括所有核小体的位置和占用，以及H3K9乙酰化和H3K4，K36，K79三甲基化。当基因组经历压缩时，核小体的组织是非常恒定的。然而，在急性减数分裂饥饿反应，核小体被重新定位，以改变选择转录起始位点的可及性。令人惊讶的是，大多数减数分裂程序没有使用这种核小体重新定位，而是由反义控制主导。组蛋白修饰状态也非常稳定，在所有基因的四分之三的特定核小体位置丰富，尽管大多数基因很少转录。我们的研究结果表明，在减数分裂过程中，基因组染色质组织的基本特征本质上是染色体的固定属性，但以受限和程序特定的方式进行调整。
The plasticity of chromatin organization as chromosomes undergo a full compendium of transactions including DNA replication, recombination, chromatin compaction, and changes in transcription during a developmental program is unknown. We generated genome-wide maps of individual nucleosome organizational states, including positions and occupancy of all nucleosomes, and H3K9 acetylation and H3K4, K36, K79 tri-methylation, during meiotic spore development (gametogenesis) in Saccharomyces. Nucleosome organization was remarkably constant as the genome underwent compaction. However, during an acute meiotic starvation response, nucleosomes were repositioned to alter the accessibility of select transcriptional start sites. Surprisingly, the majority of the meiotic programs did not use this nucleosome repositioning, but was dominated by antisense control. Histone modification states were also remarkably stable, being abundant at specific nucleosome positions at three-quarters of all genes, despite most genes being rarely transcribed. Our findings suggest that, during meiosis, the basic features of genomic chromatin organization are essentially a fixed property of chromosomes, but tweaked in a restricted and program-specific manner.