WHEAT DWARF VIRUS AC/DS VECTORS - EXPRESSION AND EXCISION OF TRANSPOSABLE ELEMENTS INTRODUCED INTO VARIOUS CEREALS BY A VIRAL REPLICON
WHEAT DWARF VIRUS AC/DS VECTORS - EXPRESSION AND EXCISION OF TRANSPOSABLE ELEMENTS INTRODUCED INTO VARIOUS CEREALS BY A VIRAL REPLICON
复制标题
DOI：
10.1073/pnas.87.19.7752
复制
发表时间：
1990-10-01
影响因子：
11.1
通讯作者：
GRONENBORN, B
中科院分区：
文献类型：
--
作者：
LAUFS, J;WIRTZ, U;GRONENBORN, B
玉米转座子激活剂（Ac）和[Ac.或解离（Ds）元件]插入禾本科植物的双生病毒，小麦矮缩病毒的基因组中不干扰病毒复制的位点。将这些重组病毒基因组导入小麦、玉米和水稻原生质体中，观察到Ac的快速有效切除。切除仅在转染后病毒可以复制的载体中检测到。这一结果并不限于自主的Ac; Ds元件的切除也诱导转座活性提供的反式表达的cDNA的Ac的质粒。这种转座子与病毒复制子的组合的植物分子遗传工程的潜力进行了讨论。
The maize transposon Activator (Ac) and in vitro-generated nonautonomous derivatives there of [Ac.DELTA. or Dissociation (Ds) elements] were inserted into the genome of a geminivirus of graminaceous plants, wheat dwarf virus, at a site that does not interfere with viral replication. These recombinant viral genomes were introduced into wheat, maize, and rice protoplasts, where rapid and efficient excision of Ac was observed. Excision was detected only in vectors in which, after transfection, the virus could replicate. This result is not restricted to the autonomous Ac; excision of Ds elements was also induced by transposes activity provided in trans by plasmids expressing the cDNA of Ac. The potential of this combination of a transposon with a viral replicon for plant molecular genetic engineering is discussed.