REGULATION OF ANTIBACTERIAL PROTEIN-SYNTHESIS FOLLOWING INFECTION AND DURING METAMORPHOSIS OF MANDUCA-SEXTA
REGULATION OF ANTIBACTERIAL PROTEIN-SYNTHESIS FOLLOWING INFECTION AND DURING METAMORPHOSIS OF MANDUCA-SEXTA
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DOI：
10.1111/j.1749-6632.1994.tb33567.x
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发表时间：
1994-01-01
期刊：
影响因子：
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通讯作者：
RUSSELL, V
中科院分区：
文献类型：
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作者：
DUNN, PE;BOHNERT, TJ;RUSSELL, V
烟草天蛾幼虫对血腔注射细菌或细菌细胞壁肽聚糖产生反应，诱导合成一系列抗菌蛋白。先前的研究已经证明肽聚糖调节这些抗菌蛋白的合成。除了引起抗菌蛋白的合成增强外，肽聚糖片段还引起"不适综合征"，其特征在于摄食和生长减少、变态延迟和排泄改变。我们推测，这些症状可能是一种机制的组成部分，以冲洗和消毒中肠腔，在昆虫的细菌感染的主要来源之一。幼稚幼虫的研究表明，溶菌酶的积累在分化蛹中肠上皮和释放到蛹中肠腔后，幼虫中肠上皮脱落的溶菌酶。这些观察结果已经通过鉴定对大肠杆菌的有效杀菌活性而得到扩展。大肠杆菌和免疫反应性血红素，以及溶菌酶，在新分化的蛹中肠腔。
Larvae of the tobacco hornworm, Manduca sexta, respond to intrahemocoelic injection of bacteria or bacterial cell wall peptidoglycan with induced synthesis of a suite of antibacterial proteins. Previous studies have demonstrated peptidoglycan regulation of the synthesis of these antibacterial proteins. In addition to eliciting enhanced synthesis of antibacterial proteins, peptidoglycan fragments also elicit a" malaise syndrome" characterized by decreased feeding and growth, delayed metamorphosis, and altered excretion. We speculate that these symptoms may be components of a mechanism to flush out and sterilize the midgut lumen, one of the primary sources of bacterial infection in insects. Studies of naive larvae have demonstrated the accumulation of lysozyme in the differentiating pupal midgut epithelium and release of lysozyme into the pupal midgut lumen after the larval midgut epithelium has been sloughed off. These observations have been extended by the identification of potent bactericidal activity against E. coli and immunoreactive hemolin, together with lysozyme, in the lumen of the newly differentiated pupal midgut.