Subunit orientation in the filamentous virus Ff(fd, f1, M13)
Subunit orientation in the filamentous virus Ff(fd, f1, M13)
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DOI：
10.1006/jmbi.1996.0323
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发表时间：
1996-06-14
影响因子：
5.6
通讯作者：
Thomas, GJ
中科院分区：
文献类型：
--
作者：
Overman, SA;Tsuboi, M;Thomas, GJ
Ff丝状病毒（fd、M13、f1）是膜蛋白组装的重要模型，并被广泛用作克隆载体和肽展示的载体。线状病毒体（约6 nm x 880 nm）包含由约2700个拷贝的50个残基的α-螺旋亚基（pVIII）包裹的单链DNA基因组。pVIII亚基从病毒体轴的平均倾斜度已经通过取向FD纤维的偏振拉曼显微光谱学实验确定，使用从肽单晶获得的酰胺I拉曼张量。发现pVIII α-螺旋和病毒体轴福尔斯之间的平均倾斜角θ落在13 <θ < 20度的范围内。(C)1996年学术出版社
The Ff filamentous viruses (fd, M13, f1) are important models for membrane protein assembly and are used extensively as cloning vectors and vehicles for peptide display The thread-like virion (approximate to 6 nm x 880 nm) comprises a single-stranded DNA genome sheathed by approximate to 2700 copies of a 50-residue alpha-helical subunit (pVIII). The average inclination of pVIII subunits from the virion axis has been determined experimentally by polarized Raman microspectroscopy of oriented fd fibers, using the amide I Raman tensors obtained from a peptide single crystal. It is found that the average tilt angle, theta, between the pVIII alpha-helix and the virion axis falls within the range 13 < theta < 20 degrees. (C) 1996 Academic Press Limited