Weighing one protein with light.
Weighing one protein with light.
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用光称量一种蛋白质。
DOI：
10.1126/science.aat5851
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Lee SF
中科院分区：
文献类型：
--
作者：
Lee SF
质谱是鉴定生物样品中蛋白质和其他生物分子的重要工具。近年来，已经报道了测量单个分子质量的方法，但是这些方法不能在真实的时间内跟踪蛋白质复合物的组装，并且仅限于相对大的蛋白质复合物。在第423页的这一问题，杨格。(1)使用光散射来检测和测量从溶液中吸附到玻璃表面的单个未标记生物分子的质量。该方法允许直接检测蛋白质，因为它们组装成功能复合物或错误折叠的聚集体。
Mass spectrometry is a crucial tool for the identification of proteins and other biomolecules in biological samples. In recent years, methods for measuring the mass of individual molecules have been reported, but these methods cannot follow the assembly of protein complexes in real time and are limited to relatively large protein complexes. On page 423 of this issue, Younget al.(1) use light scattering to detect and measure the mass of individual unlabeled biomolecules that they had adsorbed from solution to a glass surface. The method allows for the direct detection of proteins as they assemble into functional complexes or misfolded aggregates.