Long-Term Monitoring of the Physicochemical Properties of Silica-Based Nanoparticles on the Rate of Endocytosis and Exocytosis and Consequences of Cell Division
Long-Term Monitoring of the Physicochemical Properties of Silica-Based Nanoparticles on the Rate of Endocytosis and Exocytosis and Consequences of Cell Division
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DOI：
10.1080/1539445x.2012.617641
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发表时间：
2013-04-01
期刊：
影响因子：
1.2
通讯作者：
Lee, Jin-Kyu
中科院分区：
文献类型：
--
作者：
Ha, Shin-Woo;Camalier, Corinne E.;Lee, Jin-Kyu
纳米材料在尺寸、形状和电荷上是不同的，这些差异可能会改变它们在生物系统中的物理化学性质。我们已经研究了这些特性如何改变内吞作用，细胞活力，细胞分裂，胞吐作用的初始和长期动力学，以及使用基于二氧化硅的荧光纳米颗粒和鼠前成骨细胞系MC 3 T3-E1与胶原细胞外基质的相互作用。分析了三种表面改性的纳米颗粒：带正电荷（PTMA）、带负电荷（OH）和带中性电荷的聚乙二醇（PEG）。带正电荷的PTA修饰的纳米颗粒表现出最快速的吸收，在2小时内，而PEG修饰和带负电荷的OH纳米颗粒表现出较慢的吸收。细胞活力>80%，与纳米颗粒表面电荷无关，表明一般缺乏毒性。荧光强度的长期监测显示，在有丝分裂细胞分裂期间，纳米颗粒被传递到子细胞，荧光强度相应降低。这些数据表明，无论表面电荷二氧化硅纳米粒子有潜力内化到前成骨细胞，虽然有不同的动力学。此外，长寿命的纳米颗粒具有在有丝分裂期间转移到子细胞的潜力，并且可以在细胞内或在胶原基质内维持数周而没有毒性和有限的胞吐作用。
Nanomaterials are diverse in size, shape, and charge and these differences likely alter their physicochemical properties in biological systems. We have investigated how these properties alter the initial and long-term dynamics of endocytosis, cell viability, cell division, exocytosis, and interaction with a collagen extracellular matrix using silica-based fluorescent nanoparticles and the murine pre-osteoblast cell line, MC3T3-E1. Three surface modified nanoparticles were analyzed: positively charged (PTMA), negatively charged (OH), and neutrally charged polyethylene glycol (PEG). Positively charged PTMA-modified nanoparticles demonstrated the most rapid uptake, within 2 hours, while PEG modified and negatively charged OH nanoparticles demonstrated slower uptake. Cell viability was >80% irrespective of nanoparticle surface charge suggesting a general lack of toxicity. Long-term monitoring of fluorescent intensity revealed that nanoparticles were passed to daughter cells during mitotic cell division with a corresponding decrease in fluorescent intensity. These data suggest that irrespective of surface charge silica nanoparticles have the potential to internalize into pre-osteoblasts, albeit with different kinetics. Furthermore, long lived nanoparticles have the potential to be transferred to daughter cells during mitosis and can be maintained for weeks intracellularly or within a collagen matrix without toxicity and limited exocytosis.