Aureimonas galii sp nov and Aureimonas pseudogalii sp nov isolated from the phyllosphere of Galium album
Aureimonas galii sp nov and Aureimonas pseudogalii sp nov isolated from the phyllosphere of Galium album
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DOI：
10.1099/ijsem.0.001200
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发表时间：
2016-09-01
影响因子：
2.8
通讯作者：
Glaeser, Stefanie P.
中科院分区：
文献类型：
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作者：
Aydogan, Ebru L.;Busse, Hans-Juergen;Glaeser, Stefanie P.
从 Galium album 的叶圈中分离出四种黄色、革兰氏染色阴性、杆状细菌，菌株 PP-WC-4G-234(T)、PP-CE-2G-454(T)、PP-WC-1G-202 和 PP-CC-3G-650。这些菌株具有 99.7-100% 的 16S rRNA 基因序列相似性，但可以通过使用多态性 DNA PCR 的重复和随机扩增的基因组指纹来区分。基于16S rRNA基因的系统发育分析将菌株归入Aurantimonadaceae科，其16S rRNA基因序列与Aureimonas phyllosphaerae模式菌株的相似性最高，为97.2-97.3%。与所有其他橙单胞菌科的序列相似性低于 97%。该菌株的主要细胞脂肪酸是 C-18 : 1 omega 7c 作为主要脂肪酸，其次是 C-16 : 0 和汇总特征 3 (C-16 : 1 omega 7c/C-16 : 1 omega 8c)。菌株PP-WC-4G-234(T)和PP-CE-2G-454(T)的多胺谱以sym-同亚精胺为主要化合物，主要呼吸醌为泛醌Q-10。主要的极性脂质是二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰单甲基乙醇胺、磷脂酰甘油、磷脂酰胆碱、磺基喹诺酰二酰基甘油、三种未鉴定的磷脂和一种仅在总脂质染色后才能检测到的未鉴定的脂质。菌株 PP-WC-4G-234(T)、PP-CE-2G-454(T)、PP-WC-1G-202 和 PP-CC-3G-650 的 DNA G+C 含量分别为 66.4、68.9、67.4 和 70.5 mol%。基于系统发育、化学分类学和表型分析，我们提出了 Aureimonas 属的两个新物种，Aureimonas galii sp。十一月以 PP-WC-4G-234(T) (= LMG 28655(T) = CIP 110892(T)) 为模式菌株，Aureimonas fakegalii sp。十一月以 PP-CE-2G-454(T) (= LMG 29411(T) = CCM 8665(T)) 作为模式菌株，另外两个菌株代表同一物种，PP-WC-1G-202 和 PP-CC-3G-650。
Four yellow-pigmented, Gram-stain-negative, rod-shaped bacteria, strains PP-WC-4G-234(T), PP-CE-2G-454(T), PP-WC-1G-202 and PP-CC-3G-650, were isolated from the phyllosphere of Galium album. The strains shared 99.7-100% 16S rRNA gene sequence similarity but could be differentiated by genomic fingerprinting using rep-and random amplification of polymorphic DNA PCRs. Phylogenetic analysis based on the 16S rRNA gene placed the strains within the family Aurantimonadaceae with highest 16S rRNA gene sequence similarity of 97.2-97.3% to the type strain of Aureimonas phyllosphaerae. Sequence similarities to all other Aurantimonadaceae were below 97 %. The main cellular fatty acids of the strains were C-18 : 1 omega 7c as the predominant fatty acid followed by C-16 : 0 and summed feature 3 (C-16 : 1 omega 7c/C-16 : 1 omega 8c). The polyamine patterns of strains PP-WC-4G-234(T) and PP-CE-2G-454(T) contained sym-homospermidine as a major compound, and the major respiratory quinone was ubiquinone Q-10. Predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine, sulfoquinovosyldiacylglycerol, three unidentified phospholipids and one unidentified lipid only detectable after total lipid staining. The DNA G+C content was 66.4, 68.9, 67.4 and 70.5 mol% for strains PP-WC-4G-234(T), PP-CE-2G-454(T), PP-WC-1G-202 and PP-CC-3G-650, respectively. Based on phylogenetic, chemotaxonomic and phenotypic analyses we propose two novel species of the genus Aureimonas, Aureimonas galii sp. nov. with PP-WC-4G-234(T) (= LMG 28655(T) = CIP 110892(T)) as the type strain and Aureimonas pseudogalii sp. nov. with PP-CE-2G-454(T) (= LMG 29411(T) = CCM 8665(T)) as the type strain and two further strains representing the same species, PP-WC-1G-202 and PP-CC-3G-650.