Characterization of a yam class IV chitinase produced by recombinant Pichia pastoris X-33
Characterization of a yam class IV chitinase produced by recombinant Pichia pastoris X-33
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重组毕赤酵母 X-33 产生的山药 IV 类几丁质酶的表征
DOI：
10.1080/09168451.2014.885825
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
and D. Koga
中科院分区：
文献类型：
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作者：
M. A. Akond;Y. Matsuda;T. Ishimaru;K. Iwai;A. Saito;A. Kato;S. Tanaka;J. Kobayashi;and D. Koga
山药（Dioscorea oppositaThunb）IV 类几丁质酶，其基因组 DNA 由 Mitsunaga 等人克隆。 (2004)，由重组毕赤酵母X-33以高产量生产，例如66mg/L培养基。内切糖苷酶 H 处理后，通过柱层析纯化几丁质酶，然后进行表征。它显示出与 Arakane 等人报道的从山药块茎中纯化的原始几丁质酶 E 相似的特性。 （2000）。该毕赤酵母几丁质酶对尖孢镰刀菌和烟草疫霉也表现出较强的裂解活性，较宽的pH值和热稳定性，在70℃等较高温度下具有最佳活性，并且具有较高的底物亲和力，表明该毕赤酵母几丁质酶可以作为一种生防剂。
A yam (Dioscorea oppositaThunb) class IV chitinase, whose genomic DNA was cloned by Mitsunaga et al. (2004), was produced by the recombinantPichia pastorisX-33 in high yields such as 66 mg/L of culture medium. The chitinase was purified by column chromatography after Endoglycosidase H treatment and then characterized. It showed properties similar to the original chitinase E purified from the yam tuber reported by Arakane et al. (2000). ThisPichia-produced chitinase also showed strong lytic activity againstFusarium oxysporumandPhytophthora nicotianae, wide pH and thermal stability, optimum activity at higher temperature such as 70 °C, and high substrate affinity, indicating that one can use thisPichia-produced yam chitinase as a bio-control agent.