CALCITONIN ALTERS BEHAVIOR OF ISOLATED OSTEOCLASTS
CALCITONIN ALTERS BEHAVIOR OF ISOLATED OSTEOCLASTS
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DOI：
10.1002/path.1711360104
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发表时间：
1982-01-01
影响因子：
7.3
通讯作者：
MAGNUS, CJ
中科院分区：
文献类型：
--
作者：
CHAMBERS, TJ;MAGNUS, CJ
[来自大鼠的]破骨细胞在玻璃或塑料基质上孵育，观察[鲑鱼]降钙素（CT）对其行为的影响。CT前，破骨细胞活跃，细胞质向宽伪足突（板足突）后方推进，表现出强烈的褶皱活动。CT导致板足活动在几分钟内停止，随后板足逐渐碎裂和内收。CT浓度在50 pg/ml以上时，有规律地诱导破骨细胞完全静止，浓度低于10 pg/ml时，诱导的静止程度较轻。从培养基中去除CT后，这种静止状态被逆转，并被先前用胰蛋白酶治疗破骨细胞所消除。静息状态不会减少培养细胞的寿命，也不会影响它们对胰蛋白酶从玻璃中去除的抵抗力。CT未见对成骨细胞、腹腔巨噬细胞、炎性巨细胞假足活性的影响。破骨细胞静止似乎是一种可逆状态，由CT与存在于破骨细胞上的胰蛋白酶敏感CT受体相互作用诱导。诱导破骨细胞部分静止的浓度范围在人体血清浓度的生理范围内；CT显然在破骨细胞活性的调节中起生理作用。CT诱导破骨细胞的行为改变可能有助于鉴定破骨细胞的前体细胞，并有助于研究破骨的控制机制。
Osteoclasts [from rats] were incubated on a glass or plastic substrate and the effect of [salmon] calcitonin (CT) on their behavior was observed. Before exposure to CT the osteoclasts were actively motile, the cytoplasm advancing behind broad pseudopodial (lamellipodial) processes which showed intense ruffling activity. CT caused cessation of lamellipodial activity within minutes, followed by gradual fragmentation and retraction of lamellipodia. Complete osteoclast quiescence was regularly induced by concentrations of CT above 50 pg/ml, and lesser degrees of quiescence were induced at concentrations down to 10 pg/ml. This quiescent state was reversed on removing CT from the medium, and was abrogated by prior treatment of osteoclasts with trypsin. The quiescent state did not reduce the longevity of the cells in culture, nor did it affect their resistance to removal from glass by trypsin. CT showed no influence on the pseudopodial activity of osteoblasts, peritoneal macrophages or inflammatory giant cells. Osteoclast quiescence seems to be a reversible state induced by the interaction of CT with a trypsin-sensitive CT receptor, present on osteoclasts. The range of concentrations which induce partial osteoclast quiescence are within the physiological range of serum concentrations in man; CT apparently plays a physiological role in the regulation of osteoclast activity. The behavioral change induced by CT in osteoclasts may help to identify the precursor cell of the osteoclast and may assist investigations into the mechanism of control of osteoclasis.