Arrest of cultured cells in the G1 phase of the cell cycle by indomethacin.
Arrest of cultured cells in the G1 phase of the cell cycle by indomethacin.
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吲哚美辛将培养细胞抑制在细胞周期的 G1 期。
DOI：
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发表时间：
1979
影响因子：
3.5
通讯作者：
M. Beaven
中科院分区：
文献类型：
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作者：
B. Bayer;H. Kruth;M. Vaughan;M. Beaven
吲哚美辛可逆地抑制大鼠肝癌细胞(HTC)和人二倍体成纤维细胞的生长，使其处于细胞周期的G1期。细胞光度测量显示，在与消炎痛孵育48小时的细胞中，90%以上的细胞DNA含量与G1状态相对应。脱药后HTC和成纤维细胞的生长同步，表现为细胞DNA[~3H]胸腺嘧啶核苷掺入、细胞DNA含量、有丝分裂指数和细胞数的变化。HTC细胞与[~3H]胸腺嘧啶核苷孵育后的放射自显影显示，所有细胞(98%)在去掉消炎痛后参与了DNA合成。由于细胞的活力没有受到损害，即使长时间接触消炎痛，这种药物也提供了一种同步生长的方法。抑制细胞增殖可能有助于消炎痛在体内的治疗和/或毒性作用。
Indomethacin reversibly inhibited growth of rat hepatoma cells (HTC) and human diploid fibroblasts in the G1 phase of the cell cycle. Cytophotometric measurements showed that greater than 90% of cells incubated for 48 hr with indomethacin had a DNA content that corresponded to the G1 state. Synchronous growth of both the HTC and fibroblast cultures occurred after removal of drug as indicated by the sequence of changes in [3H]thymidine incorporation into DNA, cellular DNA content, mitotic index and cell number. Autoradiographs of HTC cell cultures incubated with [3H]thymidine indicated that all (98%) of the cells engaged in DNA synthesis following the removal of indomethacin. Since viability of the cells was not impaired, even by prolonged exposure to indomethacin, this drug provides a means of synchronizing growth. Suppression of cell proliferation could contribute to the therapeutic and/or toxic effects of indomethacin in vivo.