Preparation, characterization and properties of nano-hydroxyapatite/polypropylene carbonate biocomposite.
Preparation, characterization and properties of nano-hydroxyapatite/polypropylene carbonate biocomposite.
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DOI：
10.1016/j.msec.2016.02.054
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发表时间：
2016-06
期刊：
影响因子：
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通讯作者：
Jianguo Liao;Yanqun Li;Q. Zou;X. Duan;Zhengpeng Yang;Yufen Xie;Haohuai Liu
中科院分区：
文献类型：
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作者：
Jianguo Liao;Yanqun Li;Q. Zou;X. Duan;Zhengpeng Yang;Yufen Xie;Haohuai Liu
采用共沉淀法将纳米羟基磷灰石（n-HA）与聚碳酸亚丙酯（PPC）组合制备复合材料。对复合材料的物理和化学性能进行了测试。扫描电子显微镜（SEM）观察表明仿生n-HA晶体均匀分布在聚合物基体中。随着复合材料中n-HA含量的增加，复合材料的断裂机制由滑移断裂转变为滑移脆性断裂。此外，还详细研究和讨论了无机n-HA和聚碳酸亚丙酯之间的化学相互作用。 n-HA晶体的–OH/CO32 -和PPC的酯基(–COO–)之间可能形成氢键。 n-HA/PPC (40/60) 的拉伸强度与松质骨相似，达到约 58 MPa。将成骨细胞培养长达7天，然后通过甲基噻唑基四唑（MTT）比色法和SEM测量成骨细胞的粘附和增殖。细胞增殖，正常生长，呈梭形且贴壁良好。体外测试证实n-HA/PPC复合材料具有生物相容性，并且对成骨细胞显示出不可检测的负面影响。将复合材料植入新西兰白兔体内。它可以刺激新骨的生长，同时材料开始降解。这些结果表明该复合材料可能适用于骨缺损的修复或替代，并具有临床应用的潜力。
The combination of nano-hydroxyapatite (n-HA) and polypropylene carbonate (PPC) was used to make a composite materials by a coprecipitation method. The physical and chemical properties of the composite were tested. Scanning electron microscope (SEM) observation indicated that the biomimetic n-HA crystals were uniformly distributed in the polymer matrix. As the n-HA content increased in the composite, the fracture mechanism of the composites changes from gliding fracture to gliding and brittle fracture. Furthermore, the chemical interaction between inorganic n-HA and polypropylene carbonate was also investigated and discussed in detail. The hydrogen bonds might be formed between –OH/CO32 −of n-HA crystal and the ester group (–COO–) of PPC. The tensile strength of n-HA/PPC (40/60) was similar to that of the cancellous bone, and reached ca 58 MPa. The osteoblasts were cultured for up to 7 days, and then the adhesion and proliferation of osteoblasts were measured by Methyl thiazolyl tetrazolium (MTT) colorimetry assay and SEM. The cells proliferated, grew normally in fusiform shape and well attached. The in vitro test confirmed that the n-HA/PPC composites were biocompatible and showed undetectable negative effect on osteoblasts. In vivo implantation of the composite in New Zealand white rabbits was performed. It can stimulate the growth of a new bone, and at the same time the material begins to degrade. These results suggested that the composite may be suitable for the reparation or replacement of bone defects and possessed the potential for clinical applications.