Xoom: a novel oocyte membrane protein maternally expressed and involved in the gastrulation movement of Xenopus embryos.
Xoom: a novel oocyte membrane protein maternally expressed and involved in the gastrulation movement of Xenopus embryos.
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Xoom：一种新型卵母细胞膜蛋白，在母体表达并参与非洲爪蟾胚胎的原肠胚形成运动。
DOI：
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发表时间：
1999
影响因子：
0.7
通讯作者：
Tsutomu Kinoshita
中科院分区：
文献类型：
--
作者：
Kouichi Hasegawa;Toshinori Shiraishi;Tsutomu Kinoshita
在差异显示筛选非洲爪蟾胚胎中锂响应mRNA的过程中，我们发现了一个母体转录本，它被锂显着减少。分离的cDNA，命名为Xoom，编码一种新的卵母细胞膜蛋白，在N-末端的信号序列和一个单一的跨膜结构域。胞外区含有一个富含半胱氨酸的区域和一个富含丝氨酸/苏氨酸的区域，这表明与某些蛋白质的胞外关联。Xoom在卵子发生的早期在整个卵母细胞中表达，并逐渐定位于成熟卵母细胞的动物半球。合子型表达最早出现在神经褶期胚胎的背前区。此后，在神经管期胚胎的神经嵴细胞和蝌蚪的视泡和耳泡中观察到Xoom的局部表达。Xoom在成人器官中广泛表达，特别是在眼睛、心脏、肝脏和肾脏中表达水平较高。在检查Xoom和背腹图案之间的关系，锂处理在32细胞期胚胎Xoom mRNA水平下降在一个小时内，但共注射锂与肌醇逆转下降Xoom mRNA到正常水平。紫外线照射对母体Xoom的mRNA水平没有影响。过表达Xoom对发育没有影响，但反义Xoom RNA会干扰正常的原肠胚运动。这些结果表明，母体表达和膜相关的Xoom密切参与原肠胚运动通过锂诱导的信号通路。
During a process of differential display screening for lithium-responsive mRNA in Xenopus embryos, we found a maternal transcript which is remarkably reduced by lithium. The isolated cDNA, designated Xoom, encoded a novel oocyte membrane protein with a signal sequence in the N-terminus and a single transmembrane domain. The extracellular domain contained a cysteine-rich region and a serine/threonine-rich region, which suggests an extracellular association with some proteins. Expression of Xoom maternally occurred in the whole oocyte at the early stage of oogenesis and the transcript was gradually localized in the animal hemisphere of full-grown oocytes. The zygotic expression was detected at first in the dorsoanterior region of the neural fold stage embryo. Thereafter, localized expression of Xoom was observed in neural crest cells of the neural tube stage embryo and in optic and otic vesicles of tadpole. Xoom has been expressed ubiquitously in adult organs, especially with a high level in the eye, heart, liver and kidney. In examining a relation between Xoom and the dorsoventral patterning, lithium-treatment at 32-cell stage embryo decreased Xoom mRNA level within an hour, but coinjection of lithium with myo-inositol reversed the decreasing Xoom mRNA to normal level. UV-irradiation had no effect on the maternal mRNA level of Xoom. Overexpression of Xoom showed no effect on development, but antisense Xoom RNA causes interference with normal gastrulation movement. These results suggest that maternally expressed and membrane-associated Xoom is closely involved in the gastrulation movement through a lithium-inducible signal pathway.
影响因子：
10.5
作者：
Yost, C;Torres, M;Moon, RT
通讯作者：
Moon, RT
两栖动物胚胎中胚层诱导过程中肌醇磷酸盐的锂敏感生产。
DOI：
10.1126/science.1314424
发表时间：
1992
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Maslanski,JA;Leshko,L;Busa,WB
通讯作者：
Busa,WB
多磷酸肌醇循环中间体或二酰基甘油类似物可防止锂诱导的青蛙胚胎畸形发生。
影响因子：
2.7
作者：
Busa,WB;Gimlich,RL
通讯作者：
Gimlich,RL