INFORMATION-PROCESSING WITHIN THE MOTOR CORTEX .2. INTRACORTICAL CONNECTIONS BETWEEN NEURONS RECEIVING SOMATOSENSORY CORTICAL INPUT AND MOTOR OUTPUT NEURONS OF THE CORTEX
INFORMATION-PROCESSING WITHIN THE MOTOR CORTEX .2. INTRACORTICAL CONNECTIONS BETWEEN NEURONS RECEIVING SOMATOSENSORY CORTICAL INPUT AND MOTOR OUTPUT NEURONS OF THE CORTEX
复制标题
DOI：
10.1002/cne.903450203
复制
发表时间：
1994-07-08
影响因子：
2.5
通讯作者：
ASANUMA, H
中科院分区：
文献类型：
--
作者：
KANEKO, T;CARIA, MA;ASANUMA, H
通过细胞内注射biocytin和非磷酸化神经丝蛋白（npNFP）的免疫组织化学方法，研究了从第2区和第V层大锥体细胞接受体感输入的运动皮层神经元之间的联系。将生物胞素注射到运动皮层的第II/III层中的锥体细胞中，所述运动皮层对体感皮层的微刺激作出单突触和多突触反应，随后用二氨基联苯胺（DAB）和镍通过亲和素-生物素化的前氧化酶复合物方法将其染成黑色。通过使用单克隆抗体SMI-32和修改的过氧化物酶-抗过氧化物酶法与三-氨基苯甲烷（TAPM）和对甲酚作为色原，锥体细胞在运动皮层的第III和V层被染成红色的npNFP。特别是第V层的大锥体细胞贝茨细胞不仅在胞体而且在树突上都显示出强烈的npNFP免疫反应。DAB/镍和TAPM/对甲酚双重染色显示锥体细胞的生物细胞素填充的轴突曲张，这些细胞被认为接受来自第2区的单突触输入，与注射生物胞素的细胞周围的层I-III和细胞下方的层V-VI中的npNFP阳性树突接触。目前的结果表明，皮质皮质输入从2区到锥体细胞在运动皮层的第II/III层被转移到第V层锥体细胞，包括贝茨细胞，以及相邻的第II/III层锥体细胞。由于据报道，强直刺激体感皮层会在运动皮层的第II/III层细胞中产生长时程增强，因此似乎可以合理地假设体感皮层的给定区域可以在运动皮层中给定组输出细胞的活动中产生持久的变化。(C)1994 Wiley-Liss，Inc.
Connections between motor cortical neurons receiving somatosensory inputs from area 2 and large pyramidal cells in layer V were examined in the cat via intracellular injection of biocytin and immunohistochemistry of nonphosphorylated neurofilament proteins (npNFP). Biocytin was injected into pyramidal cells in layers II/III of the motor cortex that responded monosynaptically and polysynaptically to microstimulation of the somatosensory cortex and subsequently stained black by the avidin-biotinylated preoxidase complex method with diaminobenzidine (DAB) and nickel. By using a monoclonal antibody SMI-32 and a modified peroxidase-antiperoxidase method with Tris-aminophenylmethane (TAPM) and p-cresol as a chromogen, pyramidal cells in layers III and V of the motor cortex were stained red for npNFP. In particular, all the large pyramidal cells in layer V, Betz cells, displayed intense npNFP immunoreactivity not only in the perikarya but also in the dendrites.Double staining with DAB/nickel and TAPM/p-cresol showed that biocytin-filled axon varicosities of the pyramidal cells, which were thought to receive monosynaptic inputs from area 2, made contacts with npNFP-positive dendrites in layers I-III around the biocytin-injected cell and in layers V-VI beneath the cell. The present results suggest that the corticocortical input from area 2 to pyramidal cells in layers II/III of the motor cortex is transferred to layer V pyramidal cells, including Betz cells, as well as to neighboring layer II/III pyramidal cells. Since tetanic stimulation of the somatosensory cortex reportedly produces long-term potentiation in layer II/III cells of the motor cortex, it seems reasonable to assume that a given area of the somatosensory cortex can produce a long-lasting change in the activity of a given group of output cells in the motor cortex. (C) 1994 Wiley-Liss, Inc.