The stimulating effect of fatty acids and amino acid derivatives on the labellar sugar receptor of the fleshfly.
The stimulating effect of fatty acids and amino acid derivatives on the labellar sugar receptor of the fleshfly.
复制标题
DOI：
10.1085/jgp.71.1.19
复制
发表时间：
1978-01
影响因子：
3.8
通讯作者：
Shimada, I
中科院分区：
文献类型：
--
作者：
Shimada, I
7种D-氨基酸，包括D-缬氨酸、D-苯丙氨酸、D-亮氨酸、D-异亮氨酸、D-色氨酸、D-蛋氨酸和D- α -氨基丁酸，对肉蝇标签糖受体的刺激作用明显低于相应的l -异构体。len的明显效果；氨基酸侧链的结构清晰可见。-羧基的酯化和酰胺化，以及羟基和甲基的取代，导致反应极低。氨基酸的氨基位于α以外的位置几乎是无效的。由于糖受体对氨基酸的所有这些刚性立体特异性，在中性ph下，用羟基或羰基取代α -氨基会略微增加反应。一旦溶液在中性ph下缓冲，某些脂肪酸就能刺激糖受体。刺激糖受体的氨基酸和这些脂肪酸之间刺激效果的显著相似性进一步支持了这一观察结果。通过对反应浓度关系、脂肪酸衍生物的刺激作用、对氯甲苯甲酸酯的治疗效果、行为反应等方面的测试，证明了两者的相似性。
Seven D-amino acids, including D-valine, D-phenylalanine, D-leucine, D- isoleucine, D-tryptophan, D-methionine, and D-alpha-aminobutyric acid, are markedly less stimulative than the corresponding L-isomers that can stimulate the labellar sugar receptor of the fleshfly. A distinct effect of len;th of the amino acid side chain is clearly observed. Esterification and amidation of the alpha-carboxyl group, as well as substitution by hydroxyl and methyl groups, result in extremely decreased responses. Amino acids whose amino groups are located at a position other than the alpha are almost ineffective. With all these rigid stereospecificities of the sugar receptor for amino acids, certain replacement of the alpha-amino group with the hydroxyl or carbonyl group shows a slight increase of the response at neutral pH. Furthermore, certain fatty acids can stimulate the sugar receptor once the solutions are buffered at neutral pH. This observation was further supported by the presence of a remarkable similarity of stimulating effectiveness between amino acids that can stimulate the sugar receptor and those fatty acids. The similarity was shown by testing the response concentration relationships, the stimulating effect of fatty acid derivatives, the effect of treatment with p-chloromercuribenzoate, the behavioral response, and so on.