Polydatin mitigates pancreatic β-cell damage through its antioxidant activity
Polydatin mitigates pancreatic β-cell damage through its antioxidant activity
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虎杖甙通过其抗氧化活性减轻胰腺 β 细胞损伤
DOI：
10.1016/j.biopha.2020.111027
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发表时间：
2021
影响因子：
7.5
通讯作者：
Oishi Hisashi
中科院分区：
文献类型：
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作者：
Yousef Ahmed I.;Shawki Hossam H.;El-Shahawy Ahmed A.;El-Twab Sanaa M. Abd;Abdel-Moneim Adel;Oishi Hisashi
一些报道已经表明氧化应激在糖尿病及其并发症的进展中的关键作用。多叶皂苷（PD）是一种天然植物化学物质，具有广泛的药理作用，但其对胰腺的潜在有益作用尚未明确。在本研究中，我们使用体内和体外模型，研究了PD对胰腺β细胞氧化损伤的可能保护作用。给糖尿病大鼠口服PD (50 mg/kg b.w.t .) 28天后进行检查。结果显示，PD可显著提高糖尿病大鼠血液中葡萄糖耐量、胰岛素分泌及脂质代谢。有趣的是，体内实验结果表明，PD可降低脂质过氧化，改善抗氧化状态，抑制胰腺炎症。此外，我们通过将产生胰岛素的RINm5F细胞暴露于过氧化氢中，在PD存在或不存在的情况下人工诱导氧化应激。与PD共处理可保持细胞活力，减少ROS积累，增强抗氧化、抗凋亡和细胞功能标志物。由此可见，PD可能通过其抗氧化特性具有保护β细胞功能和抑制氧化损伤的作用。因此，PD有可能成为糖尿病患者的一种治疗药物。
Several reports have been shown the pivotal role of oxidative stress in the progression of diabetes mellitus and its complications. Polydatin (PD), a natural phytochemical, has wide range of pharmacological actions, however, the underlying beneficial effects in pancreas was not clarified. In the current study, usingin vivoandin vitromodels, we investigated the possible protective effects of PD against oxidative damage in pancreatic β-cells. Diabetic rats were examined after oral administration with PD (50 mg/kg b.wt.) for 28 days. Results revealed that PD significantly enhanced glucose tolerance and insulin secretion in the bloodstream of diabetic rats as well as lipid metabolism. Interestingly,in vivoresults indicated that PD decreased the lipid peroxidation, improved the antioxidant status, and inhibited the inflammation in pancreas. Alongside, we artificially induced oxidative stress by exposing the insulin-producing RINm5F cells to hydrogen peroxide in the presence or absence of PD. The co-treatment with PD preserved cell viability, reduced ROS accumulation, as well as enhanced the anti-oxidant, anti-apoptotic, and cell function markers. To conclude, PD exhibited potential action in preserving β-cell function and inhibiting oxidative damage probably through its antioxidant properties. Thus, PD could be a possible therapeutic agent for diabetic patients.