A PAPILLOMAVIRUS DNA FROM A CERVICAL-CARCINOMA AND ITS PREVALENCE IN CANCER BIOPSY SAMPLES FROM DIFFERENT GEOGRAPHIC REGIONS
A PAPILLOMAVIRUS DNA FROM A CERVICAL-CARCINOMA AND ITS PREVALENCE IN CANCER BIOPSY SAMPLES FROM DIFFERENT GEOGRAPHIC REGIONS
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DOI：
10.1073/pnas.80.12.3812
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
ZURHAUSEN, H
中科院分区：
文献类型：
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作者：
DURST, M;GISSMANN, L;ZURHAUSEN, H
来自一个宫颈浸润性癌活检样本的DNA仅在非严格条件下含有与人乳头瘤病毒（HPV）11型DNA杂交的序列。将该DNA分子克隆到λ噬菌体中。在严格的杂交条件下，它与HPV 10、14和15型交叉杂交的程度很小（小于0.1%），与其他人HPV类型的DNA没有同源性。因此，我们建议暂时将其命名为HPV 16。HPV 16 DNA被用作探针，以测试来自宫颈癌、外阴癌和阴茎癌以及良性生殖器疣（尖锐湿疣）和宫颈发育不良的其他癌症活检样本中是否存在同源序列。在61.1%（11/18）的德国患者的宫颈癌样本中，发现在高严格条件下与HPV 16 DNA杂交的序列。相比之下，来自肯尼亚和巴西的癌症活检样本中只有34.8%（8/23）显示了这种DNA。外阴癌和阴茎癌活检样本的杂交率分别为28.6%（2/7）和25%（1/4）。33例尖锐湿疣中仅有2例HPV 16 DNA阳性。这两种阳性肿瘤还含有HPV 6或HPV 11 DNA。因此，这些数据表明，HPV 16 DNA在恶性肿瘤中占主导地位，使得来自相邻乳头状瘤的乳头状瘤病毒DNA的意外污染相当不可能。除了常见的生殖器乳头状瘤病毒外，良性生殖器乳头状瘤中的罕见存在表明HPV 16复制依赖于辅助病毒。
DNA from one biopsy sample of invasive cancer of the cervix contained sequences hybridizing with human papillomavirus (HPV) type 11 DNA only under nonstringent conditions. This DNA was molecularly cloned in lambda phage. Under stringent conditions of hybridization it cross-hybridized to a minor extent (less than 0.1%) with HPV types 10, 14, and 15 and showed no homology with DNA of other human HPV types. We therefore propose to designate it tentatively as HPV 16. HPV 16 DNA was used as a probe to test additional cancer biopsy samples from cervical, vulval, and penile cancer, as well as benign genital warts (condylomata acuminata) and cervical dysplasias for the presence of homologous sequences. In 61.1% (11/18) of cervical cancer samples from German patients sequences were found hybridizing with HPV 16 DNA under conditions of high stringency. In contrast, only 34.8% (8/23) of cancer biopsy samples from Kenya and Brazil revealed this DNA. Vulval and penile cancer biopsy samples hybridized to 28.6% (2/7) or 25% (1/4), respectively. Only 2 out of 33 condylomata acuminata contained HPV 16 DNA. Both positive tumors harbored in addition HPV 6 or HPV 11 DNA. The data thus indicate that HPV 16 DNA prevails in malignant tumors, rendering an accidental contamination with papillomavirus DNA from adjacent papillomas rather unlikely. The rare presence in benign genital papillomas in addition to common genital papillomaviruses suggests a dependence of HPV 16 replication on helper virus.