An Effective and Inducible System of TAL Effector-Mediated Transcriptional Repression in Arabidopsis.
An Effective and Inducible System of TAL Effector-Mediated Transcriptional Repression in Arabidopsis.
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DOI：
10.1016/j.molp.2016.09.003
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发表时间：
2016-11
期刊：
影响因子：
27.5
通讯作者：
Sen Lin;Yuanyuan Zhao;Yingfang Zhu;Michael J. Gosney;X. Deng;Xiaohua Wang;Jinxing Lin
中科院分区：
文献类型：
--
作者：
Sen Lin;Yuanyuan Zhao;Yingfang Zhu;Michael J. Gosney;X. Deng;Xiaohua Wang;Jinxing Lin
模式生物中的基因调控对于基因功能分析至关重要，因此对于人类健康和农业生产至关重要。因此，已经开发了几种分子工具来在转录和转录后水平调节基因表达。近年来，主要存在于黄单胞菌中的转录激活因子样效应子（TALE）已被证明不仅对于基因组编辑而且对于不同生物体的基因调控也具有强大的作用（Huang et al., 2011; Cong et al., 2012; Li et al., 2012; Wang et al., 2014）。 TALE 由 N 端易位信号、中央 DNA 结合结构域、含有核定位信号的 C 端和酸性激活结构域组成 (Morbitzer et al., 2010)。 TALE DNA 结合蛋白的特异性取决于不同数量的串联排列、不完美的 34/35 氨基酸重复单元（Moscou 和 Bogdanove，2009）。每个重复的位置 12 和 13 处的重复可变二残基 (RVD) 决定了重复结构域对目标 DNA 中一个核苷酸的特异性 (Boch et al., 2009)。
Gene regulation in model organisms is critical for gene function analysis and is thus essential for human health and agricultural production. Therefore, several molecular tools have been developed to regulate gene expression at the transcriptional and post-transcriptional levels. In recent years, the transcription activator-like effector (TALE), which is predominantly found in Xanthomonas, has been proved powerful for not only genome editing but also gene regulation in different organisms (Huang et al., 2011; Cong et al., 2012; Li et al., 2012; Wang et al., 2014). TALEs are composed of an N-terminal translocation signal, a central DNA-binding domain, a C terminus containing a nuclear localization signal, and an acidic activation domain (Morbitzer et al., 2010). The specificity of TALE DNA-binding proteins depends on a variable number of tandemly arranged, imperfect 34/35-amino-acid repeat units (Moscou and Bogdanove, 2009). The repeat variable di-residues (RVD) at positions 12 and 13 of each repeat dictate the specificity of the repeat domain to one nucleotide in the targeted DNA (Boch et al., 2009).