The clinical and physiological implications of hepatoma B12-binding proteins.
The clinical and physiological implications of hepatoma B12-binding proteins.
复制标题
肝癌 B12 结合蛋白的临床和生理学意义。
作者：
S. Waxman;C. K. Liu;C. Schreiber;L. Helson
在一些肝细胞癌(肝癌)患者中发现了一种唾液酸含量增加的血清B12结合蛋白(称为肝癌B12结合蛋白)，它会导致血清B12和不饱和B12结合能力的升高。我们现在报告另一例肝癌患者，最初接近正常，不饱和B12结合能力随着疾病的进展增加了400倍，然后随着化疗的反应下降了50%。肝脏肿瘤灌流液中B12结合蛋白的含量是血清的5倍，免疫学上与从以前病例中分离的血清肝癌B12结合蛋白相同。来自肝癌的细胞系产生大量类似于肝癌的B12结合蛋白，而来自正常肝脏和其他肿瘤的细胞系则不产生。来自肝癌细胞系的肝癌血清、灌流液和培养液含有较高的唾液转移酶活性。这些数据表明，一些肝癌产生增加的超唾液酸化的B12结合蛋白，这种蛋白从血浆中缓慢清除，并在那里累积为肝癌B12结合蛋白。
A serum B12-binding protein with increased sialic acid content (termed hepatoma B12-binding protein) that causes elevations of serum B12 and unsaturated B12-binding capacity has been found in some patients with hepatocellular carcinoma (hepatoma). We now report another patient with hepatoma with initial near-normal, unsaturated B12-binding capacity that increased 400-fold as the disease progressed and then fell 50% with response to chemotherapy. A perfusate of the tumor in the liver had 5 times more B12-binding protein than did the serum and was immunologically the same as the serum hepatoma B12-binding protein isolated from previous cases. A cell line derived from hepatoma produced significant amounts of B12-binding protein similar to hepatoma B12-binding protein, whereas cell lines from normal liver and other neoplasia did not. The hepatoma sera, perfusate, and media from the hepatoma cell line contained elevated sialyltransferase activity. These data suggest that some hepatomas produce increased hypersialylated B12-binding protein that is cleared slowly from the plasma and accumulates there as hepatoma B12-binding protein.