Conditioning effects of repetitive mild neurotrauma on motor function in an animal model of focal brain injury
Conditioning effects of repetitive mild neurotrauma on motor function in an animal model of focal brain injury
复制标题
DOI：
10.1016/s0306-4522(00)00185-8
复制
发表时间：
2000-01-01
期刊：
影响因子：
3.3
通讯作者：
Esser, MJ
中科院分区：
文献类型：
--
作者：
Allen, GV;Gerami, D;Esser, MJ
采用体重下降脑损伤模型，观察反复轻度脑损伤对麻醉成年雄性SD大鼠运动功能、热休克蛋白和胶质纤维酸性蛋白表达的影响。当动物受到一系列相隔三天的三次轻微损伤时，就会产生重复性的轻微脑损伤。另一组重复性轻微损伤的动物也在最后一次轻微损伤后的三到五天内接受了随后的严重脑损伤。所有动物在手术前都接受了光束行走测试。轻度、重复轻度和重复轻度加重度脑损伤组在横梁行走试验中没有表现出运动障碍，而仅有严重脑损伤的动物在横梁行走试验中表现出显著的运动障碍(滑倒次数增加)，并在受伤后8天内恢复。重复性轻度+重度损伤和只有重度损伤的动物在后肢米皮质区域都有类似大小的皮质坏死腔。重复性轻度和重度脑损伤动物大脑皮层均有明显的热休克蛋白27 kDa和胶质纤维酸性蛋白染色。荧光JADE、热休克蛋白27 kDa和72 kDa标记表明，反复轻度脑损伤对皮质、皮质下和脊髓神经元及神经胶质细胞有广泛的影响，提示反复轻度脑损伤对大脑产生影响，因此后续同一部位的脑损伤对运动功能无影响。此外，反复的轻度损伤诱导的远离初级损伤部位的突起的激活可能在参与运动功能恢复的次级部位的激活中起作用。(C)2000年IBRO。爱思唯尔科学有限公司出版。版权所有。
A weight drop model of brain injury was used to determine the effects of repetitive mild brain injury on motor function, heat shock protein and glial fibrillary acidic protein expression in the anesthetized, adult male, Sprague-Dawley rat. Repetitive mild brain injury was produced when animals received a series of three mild injuries spaced three days apart. A separate group of repetitive mild injured animals also received a subsequent severe brain injury between three and five days after the last mild injury. All animals were trained on a beam-walking test prior to surgery. The mild, repetitive mild and repetitive mild plus severe brain injury groups showed no motor deficits in the beam-walking test, whereas the animals with only severe brain injury showed significant motor deficits (increase in number of footslips) in the beam-walking test that recovered within eight days after injury. Both repetitive mild plus severe injury and severe injury only animals had cortical necrotic cavities of similar size in the region of the hindlimb meter cortex. Both the repetitive mild and severe brain-injured animals had marked heat shock protein 27 kDa and glial fibrillary acidic protein staining in the cerebral cortex. Fluoro-Jade, heat shock protein 27 kDa and 72 kDa labeling indicated that there were widespread effects on cortical, subcortical and spinal neurons and glial cells after repetitive mild brain injury.These results suggest that repetitive mild brain injury conditions the brain so that subsequent brain injury at the same site has no effect on motor function. Furthermore, repetitive mild injury-induced activation of processes distant to the primary injury site may have a role in activation of secondary sites involved in recovery of motor function. (C) 2000 IBRO. Published by Elsevier Science Ltd. All rights reserved.