Culture and characterization of fetal human atrial and ventricular cardiac muscle cells.
Culture and characterization of fetal human atrial and ventricular cardiac muscle cells.
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胎儿人心房和心室心肌细胞的培养和表征。
DOI：
10.1007/bf02621262
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Moses,RL
中科院分区：
文献类型：
--
作者：
Claycomb,WC;Delcarpio,JB;Guice,SE;Moses,RL
从14- 18周龄胎儿心脏分离的心房和心室心肌细胞在培养中生长并表征。一旦在培养中建立，扁平细胞自发收缩，并具有分化的超微结构特征，包括有组织的肌节，闰盘和横小管与耦合。培养的心房肌细胞内可见心房颗粒。一些培养的心室肌细胞也含有与高尔基池相关的电子致密颗粒，其大小和外观与心房颗粒相似。培养的心室肌细胞分裂并表达胸苷激酶、组蛋白H4、肌球蛋白重链、肌肉特异性肌酸激酶、心房利钠因子和胰岛素样生长因子II的基因。这些结果表明，分化的人胎儿心肌细胞可以培养足够的数量进行生物化学，分子和形态学分析。
Atrial and ventricular cardiac muscle cells isolated from 14- to 18-wk old fetal human hearts were grown in culture and characterized. Once established in culture the flattened cells contracted spontaneously and possessed differentiated ultrastructural characteristics including organized sarcomeres, intercalated discs, and transverse tubules with couplings. Atrial granules were present in the cultured atrial cells. Some cultured ventricular myocytes also contained electron-dense granules associated with Golgi cisternae, which were similar in size and appearance to atrial granules. The cultured ventricular myocytes divided and expressed the genes for thymidine kinase, histone H4, myosin heavy chain, muscle-specific creatine kinase, atrial natriuretic factor, and insulin-like growth factor II. These results establish that differentiated fetal human heart muscle cells can be cultured in sufficient quantities for biochemical, molecular, and morphological analyses.