PRESENTATION AND IMMUNOGENICITY OF VIRAL EPITOPES ON THE SURFACE OF HYBRID HEPATITIS-B VIRUS CORE PARTICLES PRODUCED IN BACTERIA
PRESENTATION AND IMMUNOGENICITY OF VIRAL EPITOPES ON THE SURFACE OF HYBRID HEPATITIS-B VIRUS CORE PARTICLES PRODUCED IN BACTERIA
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DOI：
10.1099/0022-1317-71-5-1109
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发表时间：
1990-05-01
影响因子：
3.8
通讯作者：
FRANCIS, MJ
中科院分区：
文献类型：
--
作者：
CLARKE, BE;BROWN, AL;FRANCIS, MJ
我们最近报道了当肽表位作为与乙型肝炎核心抗原的融合蛋白呈现时，其免疫原性增强。在这些实验中，融合蛋白在牛痘病毒中表达。我们现在已经改进了该系统，以便可以使用简单的细菌表达系统产生大量的高免疫原性颗粒。我们将三种不同病毒表位的表达描述为嵌合颗粒，在一剂量的少量抗原后，嵌合颗粒诱导对每个表位的良好抗体反应。最后，我们证明免疫原性既反映了核心蛋白内的 T 辅助细胞位点，也反映了免疫原的颗粒性质。
We recently reported the enhanced immunogenicity of a peptide epitope when it was presented as a fusion protein with hepatitis B core antigen. In those experiments the fusion protein was expressed in vaccinia virus. We have now refined the system so that large amounts of highly immunogenic particles can be produced using a simple bacterial expression system. We describe the expression of three different viral epitopes as chimeric particles that induce good antibody responses to each epitope after one dose of low amounts of antigen. Finally, we demonstrate that the immunogenicity is a reflection of both T helper cell sites within the core protein and also the particulate nature of the immunogens.