MUTATIONS IN A NEW CHROMOSOMAL GENE OF ESCHERICHIA-COLI K-12, PCNB, REDUCE PLASMID COPY NUMBER OF PBR322 AND ITS DERIVATIVES
MUTATIONS IN A NEW CHROMOSOMAL GENE OF ESCHERICHIA-COLI K-12, PCNB, REDUCE PLASMID COPY NUMBER OF PBR322 AND ITS DERIVATIVES
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DOI：
10.1007/bf00430440
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发表时间：
1986-01-01
期刊：
影响因子：
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通讯作者：
BECKWITH, J
中科院分区：
文献类型：
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作者：
LOPILATO, J;BORTNER, S;BECKWITH, J
我们描述了使pBR322衍生物的质粒拷贝数减少的大肠杆菌突变体。对其中一个突变体进行了部分遗传学特征分析，并将其突变标记为pCnB，定位在大肠杆菌染色体上约3分钟。该基因座不同于其他基因，这些基因的产物已知会影响质粒复制或稳定的质粒维持。当pBr322以高拷贝数存在时，pcnB突变株将有助于将基因克隆到对细胞有异常或有害影响的pBr322中。
We describe mutants of Escherichia coli that decrease the plasmid copy number of pBR322 derivatives. One mutant was partially characterized genetically and its mutation, designated pcnB for plasmid copy number, was mapped to approximately 3 min on the E. coli chromosome. This locus is distinct from other genes whose products are known to affect plasmid replication or stable plasmid maintenance. The pcnB mutant strain should be useful for cloning genes into pBr322 that have aberrant or deleterious effects on the cell when present in high copy number.