Nitrogen starvation induces genome‐wide activation of transposable elements in Arabidopsis
Nitrogen starvation induces genome‐wide activation of transposable elements in Arabidopsis
复制标题
DOI：
10.1111/jipb.13376
复制
发表时间：
2022
影响因子：
11.4
通讯作者：
Qian Weiqiang
中科院分区：
文献类型：
--
作者：
Wang Yue;Liu Yi;Qu Shaofeng;Liang Wenjie;Sun Linhua;Ci Dong;Ren Zhitong;Fan Liu‐Min;Qian Weiqiang
Nitrogen (N) availability is a major limiting factor for plant growth and agricultural productivity. Although the gene regulation network in response to N starvation has been extensively studied, it remains unknown whether N starvation has an impact on the activity of transposable elements (TEs). Here, we report that TEs can be transcriptionally activated in Arabidopsis under N starvation conditions. Through genetic screening of idm1-14 suppressors, we cloned GLU1, which encodes a glutamate synthase that catalyzes the synthesis of glutamate in the primary N assimilation pathway. We found that glutamate synthase 1 (GLU1) and its functional homologs GLU2 and glutamate transport 1 (GLT1) are redundantly required for TE silencing, suggesting that N metabolism can regulate TE activity. Transcriptome and methylome analyses revealed that N starvation results in genome-wide TE activation without inducing obvious alteration of DNA methylation. Genetic analysis indicated that N starvation-induced TE activation is also independent of other well-established epigenetic mechanisms, including histone methylation and heterochromatin decondensation. Our results provide new insights into the regulation of TE activity under stressful environments in planta.
