Probing the Plant Actin Cytoskeleton during Cytokinesis and Interphase by Profilin Microinjection.
Probing the Plant Actin Cytoskeleton during Cytokinesis and Interphase by Profilin Microinjection.
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DOI：
10.1105/tpc.9.10.1815
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发表时间：
1997-10
期刊：
影响因子：
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通讯作者：
A. Valster;E. S. Pierson;R. Valenta;P. K. Hepler;A. Emons
中科院分区：
文献类型：
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作者：
A. Valster;E. S. Pierson;R. Valenta;P. K. Hepler;A. Emons
我们研究了显微注射重组桦树profilin在紫露草的分裂和间期雄蕊毛细胞的细胞学效应。在细胞分裂后期和末期微量注射profilin对胞质分裂有明显的影响，细胞板的形成往往被延迟、阻断或完全抑制。此外，细胞板的初始外观是褶皱的、薄的，有时是碎片。在间期注射profilin导致细胞质链变薄或崩溃，并以剂量依赖性方式延迟或抑制细胞质流动。共聚焦激光扫描显微镜罗丹明鬼笔环肽染色在体内发现，高水平的显微注射profilin诱导降解的肌动蛋白细胞骨架中的成膜体，核周区，和细胞质链。然而，一些皮质肌动蛋白丝保持完整。这些数据表明，profilin有能力作为一个调节因子的肌动蛋白依赖的事件，位于中央的肌动蛋白丝是更敏感的显微注射profilin比皮质肌动蛋白丝。这些结果增加了新的证据支持的假设，肌动蛋白丝在细胞板的形成中起着至关重要的作用，并提供机械支持的细胞质链在间期细胞。
We have examined the cytological effects of microinjecting recombinant birch profilin in dividing and interphase stamen hair cells of Tradescantia virginiana. Microinjection of profilin at anaphase and telophase led to a marked effect on cytokinesis; cell plate formation was often delayed, blocked, or completely inhibited. In addition, the initial appearance of the cell plate was wrinkled, thin, and sometimes fragmented. Injection of profilin at interphase caused a thinning or the collapse of cytoplasmic strands and a retardation or inhibition of cytoplasmic streaming in a dose-dependent manner. Confocal laser scanning microscopy of rhodamine-phalloidin staining in vivo revealed that high levels of microinjected profilin induced a degradation of the actin cytoskeleton in the phragmoplast, the perinuclear zone, and the cytoplasmic strands. However, some cortical actin filaments remained intact. The data demonstrate that profilin has the ability to act as a regulator of actin-dependent events and that centrally located actin filaments are more sensitive to microinjected profilin than are cortical actin filaments. These results add new evidence supporting the hypothesis that actin filaments play a crucial role in the formation of the cell plate and provide mechanical support for the cytoplasmic strands in interphase cells.