OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa)
OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa)
复制标题
OsARF12 是生长素响应基因的转录激活因子，调节根伸长并影响水稻 (Oryza sativa) 中的铁积累
DOI：
10.1111/j.1469-8137.2011.03910.x
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发表时间：
2012-01-01
期刊：
影响因子：
9.4
通讯作者：
Jiang, DeAn
中科院分区：
文献类型：
--
作者：
Qi, YanHua;Wang, SuiKang;Jiang, DeAn
生长素在维持最优根系结构(RSA)中起着重要作用，它可以应对因水分或养分短缺而导致的作物生长减速。然而，控制RSA的机制在很大程度上仍不清楚。在此，我们发现了RSA OsARF12的一个限制因子，它是一种生长素反应因子，它的敲除导致水稻的初级根长度减少。OsARF12作为转录激活剂可以促进生长素反应元件DR5：：GFP的表达，而OSA-miRNA167d通过在烟草和水稻愈伤组织中的瞬时表达抑制了OsARF12的表达。生长素浓度较低的osarf12和osarf12/25的根伸长区明显短于野生型，这可能是由于生长素合成基因OsYUCAs和生长素外流载体OsPINs和OsPGPs的表达减少所致。OsARF12基因的敲除还改变了水稻根中线粒体铁调节蛋白(OsMIR)、铁(Fe)调节转运蛋白1(OsIRT1)和胚后短根1(OsSPR1)的丰度，导致水稻根中铁含量降低，为OsARF12调控根伸长的生物学功能提供了证据。我们的研究为揭示RSA的调控以及生长素反应与铁获取之间的关系提供了新的视角。
Auxin has an important role in maintaining optimal root system architecture (RSA) that can cope with growth reductions of crops caused by water or nutrient shortages. However, the mechanism of controlling RSA remains largely unclear. Here, we found a limiting factor of RSA OsARF12 an auxin response factor whose knockout led to decreased primary root length in rice (Oryza sativa).OsARF12 as a transcription activator can facilitate the expression of the auxin response element DR5::GFP, and OsARF12 was inhibited by osa-miRNA167d by transient expression in tobacco and rice callus.The root elongation zones of osarf12 and osarf12/25, which had lower auxin concentrations, were distinctly shorter than for the wild-type, possibly as a result of decreased expression of auxin synthesis genes OsYUCCAs and auxin efflux carriers OsPINs and OsPGPs. The knockout of OsARF12 also altered the abundance of mitochondrial iron-regulated (OsMIR), iron (Fe)-regulated transporter1 (OsIRT1) and short postembryonic root1 (OsSPR1) in roots of rice, and resulted in lower Fe content.The data provide evidence for the biological function of OsARF12, which is implicated in regulating root elongation. Our investigation contributes a novel insight for uncovering regulation of RSA and the relationship between auxin response and Fe acquisition.