Direct antimicrobial susceptibility testing in bacteriuria
Direct antimicrobial susceptibility testing in bacteriuria
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DOI：
10.1111/j.1699-0463.1999.tb01578.x
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发表时间：
1999-04-01
期刊：
影响因子：
2.8
通讯作者：
Brönnestam, R
中科院分区：
文献类型：
--
作者：
Brönnestam, R
本文介绍了一种半定量培养结合直接药敏试验诊断细菌尿的方法。采用断点法进行药敏试验，采用多次接种技术。同时对发酵乳糖的肠杆菌科菌株进行固体培养基的简单生化分型和肠球菌菌株的七叶皂苷检测。葡萄球菌和链球菌采用传统的圆盘扩散法进行药敏试验。对983例连续尿培养阳性标本进行直接药敏试验和尿片扩散试验比较，882例标本6624例药敏试验结果完全一致，其中6343例（95.8%）完全一致；差异结果为特大错误15个（0.2%），特大错误84个（1.3%），小错误182个（2.7%）。直接药敏试验结果显示，与常规方法相比，肠球菌联合肠杆菌科菌尿多42例。59%的阳性尿培养在到达实验室后24小时内完成，包括药敏试验报告。诊断费用与常规方法大致相同。
A method for diagnosis of bacteriuria using semiquantitative culture in combination with direct susceptibility testing is described. Susceptibility testing with the breakpoint method was performed by means of a multiple inoculation technique. A simple biochemical typing on solid media of lactose-fermenting Enterobacteriaceae strains and aesculin testing of Enterococcus strains were performed simultaneously. Staphylococcus and Streptococcus strains were tested for susceptibility using the conventional disc diffusion technique. The results of the direct susceptibility test and the disc diffusion test were compared in a study of 983 consecutively positive urine cultures: 6624 susceptibility tests from 882 specimens with one microorganism revealed complete agreement in 6343 cases (95.8%); the discrepant results were 15 very major errors (0.2%), 84 major errors (1.3%) and 182 minor errors (2.7%). The direct susceptibility test revealed 42 more cases of bacteriuria with enterococci in combination with Enterobacteriaceae strains compared to the conventional method. 59% of all positive urine cultures were completed, including the susceptibility test report, within 24 h after arriving at the laboratory. The diagnostic costs were approximately the same as for the conventional method.