Phylogeography of the greater horseshoe bat, Rhinolophus ferrumequinum: contrasting results from mitochondrial and microsatellite data
Phylogeography of the greater horseshoe bat, Rhinolophus ferrumequinum: contrasting results from mitochondrial and microsatellite data
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DOI：
10.1111/j.1365-294x.2008.04021.x
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发表时间：
2009-01-01
影响因子：
4.9
通讯作者：
Rossiter, Stephen J.
中科院分区：
文献类型：
--
作者：
Flanders, Jon;Jones, Gareth;Rossiter, Stephen J.
系统地理研究通常基于单倍型数据，偶尔也基于微卫星等核标记，但很少将两者结合起来。这是不幸的，因为使用遗传模式和进化速度不同的标记可能会更准确和全面地了解物种的历史。在这里，我们详细地研究了大马蹄蝙蝠的系统地理，利用来自其古北界45个地点的1098个核苷酸的线粒体ND2基因来推断种群历史。此外，我们重新分析了可用于该物种的大型微卫星数据集，并比较了两个标记的结果，以推断种群关系和影响它们的历史过程。我们发现，在系统地理学研究中最受欢迎的标记mtDNA产生了一个误导性的结果，并将导致我们错误地得出欧洲发生了单一扩张的结论。只有将线粒体和微卫星数据集结合起来，我们才能重建物种的历史，并展示欧洲的两次殖民事件，一次是在最后一次冰川高峰期(LGM)之前，另一次是在LGM之后。结合标记还揭示了小亚细亚作为该物种的古代避难所的重要性，以及在末次盛会之前将大马蹄蝙蝠扩展到欧洲的来源种群。
Phylogeographical studies are typically based on haplotype data, occasionally on nuclear markers such as microsatellites, but rarely combine both. This is unfortunate because the use of markers with contrasting modes of inheritance and rates of evolution might provide a more accurate and comprehensive understanding of a species' history. Here we present a detailed study of the phylogeography of the greater horseshoe bat, Rhinolophus ferrumequinum, using 1098 bp of the mitochondrial ND2 gene from 45 localities from across its Palaearctic range to infer population history. In addition, we re-analysed a large microsatellite data set available for this species and compared the results of both markers to infer population relationships and the historical processes influencing them. We show that mtDNA, the most popular marker in phylogeography studies, yielded a misleading result, and would have led us to conclude erroneously that a single expansion had taken place in Europe. Only by combining the mitochondrial and microsatellite data sets are we able to reconstruct the species' history and show two colonization events in Europe, one before the Last Glacial Maximum (LGM) and one after it. Combining markers also revealed the importance of Asia Minor as an ancient refugium for this species and a source population for the expansion of the greater horseshoe bat into Europe before the LGM.