The anaerobic survival mechanism of Schizophyllum commune 20R-7-F01, isolated from deep sediment 2 km below the seafloor.
The anaerobic survival mechanism of Schizophyllum commune 20R-7-F01, isolated from deep sediment 2 km below the seafloor.
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DOI：
10.1111/1462-2920.15332
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发表时间：
2020-11
影响因子：
5.1
通讯作者：
Muhammad Zain ul Arifeen;C. Chu;Xinyi Yang;Junzhong Liu;Xin Huang;Yunan Ma;Xuan Liu;Ya-rong Xue;Changhong Liu
中科院分区：
文献类型：
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作者：
Muhammad Zain ul Arifeen;C. Chu;Xinyi Yang;Junzhong Liu;Xin Huang;Yunan Ma;Xuan Liu;Ya-rong Xue;Changhong Liu
真菌在洋底以下的厌氧沉积环境中占主导地位，在那里它们发挥着重要的生态作用。然而，真菌对这些厌氧环境的适应机制仍不清楚。本文通过生化、代谢组学和转录组学分析，研究了裂褶菌（Schizophyllum commune）20 R-7-F01的厌氧适应机制。在完全缺氧条件下，菌丝生长良好，但形态变化明显，子实体发育不完全。与好氧条件相比，该菌在厌氧条件下支链氨基酸（BCAAs）生物合成和乙醇发酵能力增强，与这些代谢相关的基因表达显著上调。此外，该真菌显示了通过利用糖酵解和乙醇发酵途径合成乙醇的新策略。这些发现表明，海底真菌可能采取多种机制来科普缺氧。本文受版权保护。All rights reserved.
Fungi dominated the eukaryotic group in the anaerobic sedimentary environment below the ocean floor where they play an essential ecological role. However, the adaptive mechanism of fungi to these anaerobic environments is still unclear. Here, we reported the anaerobic adaptive mechanism of Schizophyllum commune 20R-7-F01, isolated from deep coal-bearing sediment down to ~2 km below the seafloor, through biochemical, metabolomic and transcriptome analyses. The fungus grows well, but the morphology changes obviously and the fruit body develops incompletely under complete hypoxia. Compared with aerobic conditions, the fungus has enhanced branched-chain amino acid (BCAAs) biosynthesis and ethanol fermentation under anaerobic conditions, and genes related to these metabolisms have been significantly up-regulated. Additionally, the fungus shows novel strategies for synthesizing ethanol by utilizing both glycolysis and ethanol fermentation pathways. These findings suggest that the subseafloor fungi may adopt multiple mechanisms to cope with lack of oxygen. This article is protected by copyright. All rights reserved.