CD8+TCR+ and CD8+TCR- cells in whole bone marrow facilitate the engraftment of hematopoietic stem cells across allogeneic barriers
CD8+TCR+ and CD8+TCR- cells in whole bone marrow facilitate the engraftment of hematopoietic stem cells across allogeneic barriers
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DOI：
10.1016/s1074-7613(00)80133-8
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发表时间：
1999-11-01
期刊：
影响因子：
32.4
通讯作者：
Weissman, IL
中科院分区：
文献类型：
--
作者：
Gandy, KL;Domen, J;Weissman, IL
虽然纯化的造血干细胞（HSC）足以植入辐射同种异体受体，骨髓（BM）含有其他细胞，促进植入。在这里，几个候选的促进者进行了测试，与HSC的共移植。TCR+和TCR（-）CD 8 α（+）BM亚群均具有促进潜力。CD 8（+）TCR（+）细胞是典型的T淋巴细胞。CD 8（+）TCR（-）易化因子是CD 3（-），而不是CD 3（+），具有颗粒状形态，是CD 8 β（-）和CD 11 c（+）;它们与CD 8 α α淋巴树突细胞和否决细胞共享表型特征。我们还表明，裂解功能是不必要的促进和CD 8 α分子是重要的促进或在发展中的调解人。
Although purified hematopoietic stem cells (HSC) are sufficient to engraft irradiated allogeneic recipients, bone marrow (BM) contains other cells that facilitate engraftment. Here, several candidate facilitators were tested by cotransplantation with HSC. Both TCR+ and TCR(-)CD8 alpha(+) BM subpopulations have facilitative potential. CD8(+)TCR(+) cells are typical T lymphocytes. CD8(+)TCR(-) facilitators are CD3(-), not CD3(+), have a granular morphology, and are CD8 beta(-) and CD11c(+); they share phenotypic characteristics with CD8 alpha alpha lymphoid dendritic cells and veto cells. We also demonstrate that lytic function is not necessary for facilitation and that the CD8 alpha molecule is either important for facilitation or in the development of facilitators.