Analysis of the role of mitochondrial and endoplasmic reticulum localized small heat shock proteins in tomato
Analysis of the role of mitochondrial and endoplasmic reticulum localized small heat shock proteins in tomato
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DOI：
10.1007/s10535-010-0127-7
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发表时间：
2010-09
影响因子：
1.5
通讯作者：
P. Nautiyal;M. Shono
中科院分区：
文献类型：
--
作者：
P. Nautiyal;M. Shono
本通讯研究了针对线粒体(MT)和内质网(ER)的小分子热休克蛋白(SHsps)在番茄植株(LycopersicesculentumMill.)在热应激下。转基因植株和野生型植株在适温、中温和高温下的遗传反应不同。在最适温度下，野生型的生物量高于转基因品系，而在中等温度下，mt-SHSP品系的生物量有所增加。此外，mt-SHSP品系的净光合速率(Pn)在两种温度升高时都有所增加，但在中等温度升高时增加幅度更大。高温处理后，所有转基因品系的细胞膜稳定性(CMS)均有所改善，但转基因品系始终保持较高的细胞膜稳定性。转基因株系在不同温度条件下营养和生殖部位均表达sHsps，而野生型仅在热激1h后表达。
This communication examines the role of small heat shock proteins (sHsps) targeted to mitochondria (Mt) and endoplasmic reticulum (ER) in tomato plants (Lycopersicon esculentumMill.) under heat stress. Genetic response of transgenic and wild type plants varied under optimum, moderately elevated and elevated temperature. In optimum temperature higher biomass was recorded in wild type than the transgenic lines, whereas in moderately elevated temperature biomass increased in Mt-sHsp line. Also, net photosynthetic rate (PN) increased in Mt-sHsp line in both the elevated temperatures, though higher in moderately elevated. Cell membrane stability (CMS) improved in all the lines after exposure to elevated temperatures, but always remained higher in transgenic lines. Transgenic lines expressed sHsps in different temperature regimes in both vegetative and reproductive parts, while wild type expressed such proteins only after 1 h of heat shock.