Fluorescence studies of chlorophyll a incorporated into lipid mixtures, and the interpretation of "phase" diagrams.
Fluorescence studies of chlorophyll a incorporated into lipid mixtures, and the interpretation of "phase" diagrams.
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掺入脂质混合物中的叶绿素 a 的荧光研究以及“相”图的解释。
DOI：
10.1016/0005-2736(75)90054-1
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
A. Lee
中科院分区：
文献类型：
--
作者：
A. Lee
报道了磷脂酰胆碱和磷脂酰乙醇胺混合脂质体中氯化吡啶α的荧光性质。荧光强度与温度的关系曲线显示了在特征温度下的断裂，这可以归因于固相脂质形成的开始和完成。这些温度可以绘制成类似于宏观体系相图的图示。讨论了由“小系统效应”引起的复杂性，并将实验图与理想混合计算的理论相图进行了比较。胆固醇的引入导致荧光强度降低，最容易用A1：1脂：胆固醇相互作用和单体、荧光、叶绿素的排除来解释。二价离子与二棕榈酰磷脂酰胆碱和二棕榈酰磷脂酰丝氨酸的混合物相互作用，导致叶绿素从磷脂酰丝氨酸中排除。
The fluorescence of chloropyll α incorporated into liposomes of mixtures of phosphatidylcholines and phosphatidylethanolamines is reprted. Plots of fluorescence intensities against temperature show breaks at characteristic temperatures which can be attributed to the onset and completion of solid phase lipid formation. These temperatures can be plotted to give diagrams analogous to the phase diagrams obtained for macroscopic systems. Complications due to “small-system effects” are discussed, and the experimental diagrams are compared with theoretical phase digrams calculated for ideal mixing. Introduction of cholesterol leads to a reduction in fluorescence intensity, most readily explained by a1 : 1 lipid :cholesterol interaction with exclution of monomeric, fluorescent, chlorophylla. Interaction of divalent ions with mixtures of dipalmitoyl phosphatidylcholine and dipalmitoyl phosphatidylserine leads to exclution oc chlorophyllafrom the phosphatidylserine.