BOC, an Ig superfamily member, associates with CDO to positively regulate myogenic differentiation
BOC, an Ig superfamily member, associates with CDO to positively regulate myogenic differentiation
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DOI：
10.1093/emboj/21.1.114
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发表时间：
2002-01-15
期刊：
影响因子：
11.4
通讯作者：
Krauss, RS
中科院分区：
文献类型：
--
作者：
Kang, JS;Mulieri, PJ;Krauss, RS
CDO是一种细胞表面受体样蛋白，积极调节肌源性分化。本文报道了BOC的鉴定，它与CDO一起定义了免疫球蛋白超家族中一个新认识的亚家族。在发育中的小鼠胚胎中，Cdo和boc在肌肉前体中共表达。与CDO一样，BOC加速了培养成肌细胞系的分化，并参与了肌源性转录因子MyoD的正反馈循环。CDO和BOC通过其胞外结构域和胞内结构域的结合以顺式方式形成复合物。含有整个BOC外结构域的可溶性融合蛋白的功能与全长BOC相似，可以促进肌源性分化，这表明细胞内区域对于其在该系统中的活性是不可或缺的。此外，显性阴性形式的CDO抑制可溶性BOC的促肌作用，表明BOC依赖于CDO的活性。CDO和BOC被认为是受体复合物的组成部分，介导肌肉前体之间的一些细胞-细胞相互作用，这是肌肉形成所必需的。
CDO is a cell surface receptor-like protein that positively regulates myogenic differentiation. Reported here is the identification of BOC, which, with CDO, defines a newly recognized subfamily within the immunoglobulin superfamily. cdo and boc are co-expressed in muscle precursors in the developing mouse embryo. Like CDO, BOC accelerates differentiation of cultured myoblast cell lines and participates in a positive feedback loop with the myogenic transcription factor, MyoD. CDO and BOC form complexes in a cis fashion via association of both their ectodomains and their intracellular domains. A soluble fusion protein that contains the entire BOC ectodomain functions similarly to full-length BOC to promote myogenic differentiation, indicating that the intracellular region is dispensable for its activity in this system. Furthermore, a dominant-negative form of CDO inhibits the pro-myogenic effects of soluble BOC, suggesting that BOC is dependent on CDO for its activity. CDO and BOC are proposed to be components of a receptor complex that mediates some of the cell-cell interactions between muscle precursors that are required for myogenesis.