Susceptibility to Leishmania major of different populations of Phlebotomus papatasi (Diptera: Psychodidae) from endemic and non-endemic regions of Egypt
Susceptibility to Leishmania major of different populations of Phlebotomus papatasi (Diptera: Psychodidae) from endemic and non-endemic regions of Egypt
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DOI：
10.1080/00034989860175
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发表时间：
1998-01-01
影响因子：
--
通讯作者：
Tetreault, GE
中科院分区：
文献类型：
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作者：
Hanafi, HA;El Sawaf, BM;Tetreault, GE
在实验室中评估了从埃及不同地区（西奈、阿斯旺和三角洲地区）收集的三个帕帕塔西白蛉种群成功获得和传播硕大利什曼原虫（西奈白蛉分离株IPAP/EG/89/SI-177）的能力。每个种群的苍蝇都用感染的血液悬浮液人工喂养，使用鸡皮膜喂养装置，自然地用感染的小鼠喂养。解剖并显微镜检查喂食的苍蝇样品，以确定寄生虫感染的感染率和强度。阿斯旺种群的摄食率（16.2%）显著低于西奈（51.2%）和三角洲（69.7%）种群（P < 0.0001）。西奈人口的感染率（65.9%）显著高于三角洲人口的感染率（52.3%; P < 0.05），略高于阿斯旺人口的感染率（62.5%）。L的强度没有观察到差异。主要感染中肠的白蛉检查从任何三个种群。用感染L.主要，三个种群的摄食率显示出类似的模式，在膜饲料中看到的，与阿斯旺人口具有最低的利率。在每个种群的两个单独试验中，将一组人工感染的苍蝇喂给未感染的BALB/c小鼠。在西奈种群中暴露于受感染的白蛉后36天，在一只动物的嘴角上观察到利什曼病病变。这些观察结果提供了帕帕塔斯是L.埃及的少校
The ability of three populations of Phlebotomus papatasi collected from different areas of Egypt (Sinai, Aswan and Delta regions) to acquire successfully and transmit Leishmania major (Sinai sandfly isolate IPAP/EG/89/SI-177) was evaluated in the laboratory. Flies from each population were fed artificially on infected blood suspensions, using a chick-skin-membrane feeding apparatus, and naturally on infected mice. Samples of fed flies were dissected and examined microscopically to determine the infection rate and intensity of parasite infection. The Aswan population had a significantly lower feeding rate (16.2%) than the Sinai (51.2%) and Delta (69.7%) populations (P < 0.0001). The infection rate for the Sinai population was significantly higher (65.9%) than the rate for the Delta (52.3%; P < 0.05) and slightly higher than that for the Aswan (62.5%). No differences were observed in the intensity of L. major infection in the midguts of the sandflies examined from any of the three populations. When flies from each population were fed naturally on BALB/c mice infected with L. major, the feeding rates of the three populations showed a similar pattern to that seen in the membrane feeds, with the Aswan population having the lowest rate. In each of two separate trials for each population, a group of artificially infected flies was refed on uninfected BALB/c mice. Thirty-six days following exposure to the infected sandflies in the Sinai population, a leishmanial lesion was observed on the corner of one animal's mouth. These observations provide the final piece of evidence that P. papatasi is a vector of L. major in Egypt.