Physical Model of the Dynamic Instability in an Expanding Cell Culture
Physical Model of the Dynamic Instability in an Expanding Cell Culture
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DOI：
10.1016/j.bpj.2009.10.022
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发表时间：
2010-02-03
影响因子：
3.4
通讯作者：
Silberzan, Pascal
中科院分区：
文献类型：
--
作者：
Mark, Shirley;Shlomovitz, Roie;Silberzan, Pascal
细胞集体迁移在许多生物过程中具有重要意义。这项工作的目的是为伤口愈合反应过程中细胞迁移的动力学提供一个物理模型。实验表明，最初均匀的细胞培养单层以不均匀的方式扩展，形成手指状。这些手指状的细胞培养前沿是由集体移动的细胞柱组成的。我们提出了一个物理模型来解释这种现象，基于动态不稳定性的概念。在这个模型中，我们将移动细胞培养物前面的第一层细胞视为一个连续的一维膜（轮廓），具有膜的通常弹性：曲率和表面张力。这种膜是活跃的，由于细胞的细胞运动力，我们认为这种运动性与培养界面的局部曲率有关；较大的凸曲率与较强的细胞运动力相关。这种形状-力关系引起了动态不稳定性，然后我们将其与伤口愈合实验中观察到的模式进行比较。
Collective cell migration is of great significance in many biological processes. The goal of this work is to give a physical model for the dynamics of cell migration during the wound healing response. Experiments demonstrate that an initially uniform cell-culture monolayer expands in a nonuniform manner, developing fingerlike shapes. These fingerlike shapes of the cell culture front are composed of columns of cells that move collectively. We propose a physical model to explain this phenomenon, based on the notion of dynamic instability. In this model, we treat the first layers of cells at the front of the moving cell culture as a continuous one-dimensional membrane (contour), with the usual elasticity of a membrane: curvature and surface-tension. This membrane is active, due to the forces of cellular motility of the cells, and we propose that this motility is related to the local curvature of the culture interface; larger convex curvature correlates with a stronger cellular motility force. This shape-force relation gives rise to a dynamic instability, which we then compare to the patterns observed in the wound healing experiments.