Toward an early diagnosis of lung cancer: An autoantibody signature for squamous cell lung carcinoma
Toward an early diagnosis of lung cancer: An autoantibody signature for squamous cell lung carcinoma
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DOI：
10.1002/ijc.23680
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发表时间：
2008-10-01
影响因子：
6.4
通讯作者：
Meese，Eckart
中科院分区：
文献类型：
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作者：
Leidinger，Petra;Keller，Andreas;Meese，Eckart
基于血清的诊断为早期肺癌检测和CT鉴别良恶性结节提供了前景。未来基于血液的诊断的一个主要挑战在于显示血清反应性模式不仅可以将肺癌患者与正常对照区分开来，还可以将其与非肿瘤肺部病变患者区分开来。我们解决了鳞状细胞肺癌，最常见的肺肿瘤类型之一的这个问题。使用一组82个表达潜在自身抗原的噬菌体肽克隆，我们进行了血清学斑点试验。我们筛选了108份血清，包括39份来自鳞状细胞肺癌患者的血清，29份来自其他非肿瘤肺部病变患者的血清，以及40份来自无已知疾病的志愿者的血清。为了对血清组进行分类，我们采用了标准的朴素贝叶斯方法结合子集选择方法。我们能够区分鳞状细胞肺癌和正常血清，准确率为93%。低级别鳞状细胞肺癌与正常血清分离，准确率为92.9%。我们能够区分鳞状细胞肺癌与非肿瘤肺部病变，准确率为83%。与ROCK 1、PRKCB 1和KIAA0376具有序列同源性的三个噬菌体肽克隆与超过15%的癌症血清反应，但既不与正常血清反应，也不与非肿瘤肺病理血清反应。我们的研究表明，血清反应性谱结合统计分类方法具有很大的潜力，不仅可以将鳞状细胞肺癌患者与正常对照区分开来，还可以将其与非肿瘤肺部病变患者区分开来。© 2008 Wiley利斯公司
Serum‐based diagnosis offers the prospect of early lung carcinoma detection and of differentiation between benign and malignant nodules identified by CT. One major challenge toward a future blood‐based diagnostic consists in showing that seroreactivity patterns allow for discriminating lung cancer patients not only from normal controls but also from patients with non‐tumor lung pathologies. We addressed this question for squamous cell lung cancer, one of the most common lung tumor types. Using a panel of 82 phage‐peptide clones, which express potential autoantigens, we performed serological spot assay. We screened 108 sera, including 39 sera from squamous cell lung cancer patients, 29 sera from patients with other non‐tumor lung pathologies, and 40 sera from volunteers without known disease. To classify the serum groups, we employed the standard Naïve Bayesian method combined with a subset selection approach. We were able to separate squamous cell lung carcinoma and normal sera with an accuracy of 93%. Low‐grade squamous cell lung carcinoma were separated from normal sera with an accuracy of 92.9%. We were able to distinguish squamous cell lung carcinoma from non‐tumor lung pathologies with an accuracy of 83%. Three phage‐peptide clones with sequence homology toROCK1, PRKCB1andKIAA0376reacted with more than 15% of the cancer sera, but neither with normal nor with non‐tumor lung pathology sera. Our study demonstrates that seroreactivity profiles combined with statistical classification methods have great potential for discriminating patients with squamous cell lung carcinoma not only from normal controls but also from patients with non‐tumor lung pathologies. © 2008 Wiley‐Liss, Inc.