Fatty acids selectively inhibit eukaryotic DNA polymerase activities in vitro
Fatty acids selectively inhibit eukaryotic DNA polymerase activities in vitro
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DOI：
10.1016/0167-4781(96)00121-2
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发表时间：
1996-09-11
期刊：
影响因子：
--
通讯作者：
Sakaguchi, K
中科院分区：
文献类型：
--
作者：
Mizushina, Y;Tanaka, N;Sakaguchi, K
研究了真核 DNA 聚合酶和脂肪酸之间的体外关系。一些脂肪酸在体外强烈抑制DNA聚合酶α和/或β的活性。亚油酸的抑制动力学表明，DNA 聚合酶 α 相对于 DNA 模板和底物 (dTTP) 受到非竞争性抑制，而 DNA 聚合酶 β 则受到 DNA 和底物的竞争性抑制。
The in vitro relationship between eukaryotic DNA polymerases and fatty acids was investigated. Some fatty acids strongly inhibited the activities of DNA polymerase alpha and/or beta in vitro. The kinetics of inhibition by linoleic acid showed that DNA polymerase alpha was non-competitively inhibited with respect to the DNA template and substrate (dTTP), while DNA polymerase beta was inhibited competitively with both DNA and substrate.