High glucose exacerbates neutrophil adhesion to human retinal endothelial cells
High glucose exacerbates neutrophil adhesion to human retinal endothelial cells
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DOI：
10.1016/j.exer.2005.08.022
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发表时间：
2006-01-01
影响因子：
3.4
通讯作者：
Ogura, Y
中科院分区：
文献类型：
--
作者：
Hirata, F;Yoshida, M;Ogura, Y
白细胞在视网膜上的积累被认为在糖尿病视网膜病变的早期阶段起着关键作用。白细胞内皮粘附对白细胞停滞很重要，高葡萄糖水平可能影响它。然而，很少有研究表明白细胞-内皮细胞在高糖暴露后使用培养的人视网膜内皮细胞（HRECs）粘附。我们在此报告高糖暴露后中性粒细胞内皮粘附测定的结果。与对照组相比，高浓度葡萄糖（46.1 mM持续48小时）显著增加了中性粒细胞对HRECs的粘附。这种现象被PKC抑制剂和抗细胞间粘附分子-1抗体阻断。高渗透压不影响中性粒细胞与内皮细胞的粘附。这些结果表明，葡萄糖通过激活PKC途径，通过ICAM-1的表面表达增强中性粒细胞对HRECs的粘附。(C) 2005 Elsevier Ltd版权所有。
Leukocyte accumulation in the retina is thought to play a key role in the earl), stage of diabetic retinopathy. Leukocyte-endothelial adhesion is important for leukostasis, and high glucose levels may influence it. However, few studies have demonstrated leukocyte-endothelial adhesion using cultured human retinal endothelial cells (HRECs) after high glucose exposure. We report herein the results of neutrophil-endothelial adhesion assay after high glucose exposure. A high concentration of glucose (46.1 mM for 48 h) significantly increased neutrophil adhesion to HRECs as compared to controls. This phenomenon was blocked by PKC inhibitor and anti-intercellular adhesion molecule-1 antibody. Hyperosmolarity did not affect neutrophil-endothelial adhesion. These results suggested that glucose enhances neutrophil adhesion to HRECs through surface expression of ICAM-1 via activation of the PKC pathway. (C) 2005 Elsevier Ltd. All rights reserved.