Detection and analysis of iron uptake components expressed by Acinetobacter baumannii clinical isolates
Detection and analysis of iron uptake components expressed by Acinetobacter baumannii clinical isolates
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DOI：
10.1128/jcm.41.9.4188-4193.2003
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发表时间：
2003-09-01
影响因子：
9.4
通讯作者：
Actis, LA
中科院分区：
文献类型：
--
作者：
Dorsey, CW;Beglin, MS;Actis, LA
鲍氏不动杆菌19606原型菌株产生与FatA免疫相关的78 kDa铁调节外膜蛋白，FatA是鱼类病原体鳗弧菌通过anguibactin介导的系统获得铁所需的。这个A.鲍曼不动杆菌菌株还分泌组胺，组胺是铁载体anguibactin的生物合成前体。相比之下，A.分离自俄勒冈州的鲍曼不动杆菌8399临床菌株产生的铁载体和推定的73-kDa铁调节外膜（OM 73）受体不同于由鳗弧菌和A. baumannii 19606.这些观察结果表明，不同的A.鲍曼不动杆菌临床分离株表达不相关的铁摄取系统。这一假设得到了不同的A.从俄勒冈州和欧洲获得的鲍曼不动杆菌分离物。19606分离株和一些欧洲分离株表达FatA样蛋白，而19606和任何欧洲分离株都不表达与OM 73相关的蛋白。一些欧洲分离株未能表达FatA和OM 73样蛋白。除了一个俄勒冈州分离株外，所有分离株都表达了OM 73样蛋白，而没有一个分离株含有FATA样蛋白。这些蛋白质的存在总是与同源菌株中的om 73和fatA样基因的存在相关。虽然19606和一些欧洲分离株产生组胺，但俄勒冈州分离株均不具有这种能力。有趣的是，来自俄勒冈州和欧洲分离株的各一个菌株不表达任何参与铁获取的这些产物，表明它们可以通过铁载体介导的转运系统获取铁，该转运系统不同于19606和8399临床分离株所表达的转运系统。
The Acinetobacter baumannii 19606 prototype strain produces a 78-kDa iron-regulated outer membrane protein immunologically related to FatA, which is required for iron acquisition by the fish pathogen Vibrio anguillarum via the anguibactin-mediated system. This A. baumannii strain also secretes histamine, a biosynthetic precursor of the siderophore anguibactin. In contrast, the A. baumannii 8399 clinical strain isolated in Oregon produces a siderophore and a putative 73-kDa iron-regulated outer membrane (OM73) receptor that are different from those produced by V. anguillarum and A. baumannii 19606. These observations suggest that different A. baumannii clinical isolates express unrelated iron uptake systems. This hypothesis is supported by differences in outer membrane protein profiles among A. baumannii isolates obtained from Oregon and Europe. The 19606 isolate and some European isolates expressed a FatA-like protein, while neither 19606 nor any of the European isolates expressed proteins related to OM73. Some European isolates failed to express FatA- and OM73-like proteins. All but one of the Oregon isolates expressed OM73-like proteins, while none of them contained a FatA-like protein. The presence of these proteins always correlated with the presence of the om73- and fatA-like genes in the cognate strains. While 19606 and a few European isolates produced histamine, none of the Oregon isolates had this capability. Interestingly, one strain each from the Oregon and European isolates did not express any,of these products involved in iron acquisition, indicating that they could acquire iron through siderophore-mediated transport systems different from those expressed by the 19606 and 8399 clinical isolates.