Progesterone induces expression of Lrp2 in the murine uterus.
Progesterone induces expression of Lrp2 in the murine uterus.
复制标题
黄体酮诱导小鼠子宫中 Lrp2 的表达。
DOI：
10.1016/j.bbrc.2013.10.037
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发表时间：
2013
影响因子：
3.1
通讯作者：
Jeong,Jae-Wook
中科院分区：
文献类型：
--
作者：
Oh,SeoJin;Kim,TaeHoon;Lim,JeongMook;Jeong,Jae-Wook
孕酮(P4)和孕激素受体(PR)在子宫环境中具有重要作用。在以往的研究中，我们利用高密度DNA芯片分析，我们发现低密度脂蛋白受体相关蛋白2(Lrp2)是P4和PR上调的基因之一。在本研究中，我们通过实时定量聚合酶链式反应、原位杂交和免疫组织化学方法检测了Lrp2在野生型小鼠子宫腔上皮和腺上皮中的表达。然而，在P4处理的孕激素受体基因敲除(PRKO)小鼠中，没有观察到Lrp2的表达。Lrp2的表达不受雌激素的调节。在妊娠早期，Lrp2在子宫腔上皮和腺上皮中的表达在2.5dpc时检测到，然后在3.5dpc时显著增加。这些结果表明，Lrp2是一个新的P4和PR靶基因。
Progesterone (P4) and progesterone receptor (PR) have important functions in uterine environment. In previous studies, using high density DNA microarray analysis, we identified low density lipoprotein receptor-related protein 2 (Lrp2) is one of the genes upregulated by P4 and PR. In present studies, we examined the expression ofLrp2through real-time PCR,in situhybridization and immunohistochemistry by P4-PR response.Lrp2mRNA transcript was significantly increased after P4 treatment in the luminal and glandular epithelium of the wild-type mice. However,Lrp2expression was not observed in the progesterone receptor knock out (PRKO) mice treated with P4. The expression ofLrp2expression is not regulated by estrogen. During early pregnancy, the expression ofLrp2was detected at 2.5 dpc and then significantly increased at 3.5 dpc in luminal and glandular epithelium. These results suggest thatLrp2is a novel target gene by P4 and PR.