Characterization of novel proteins affected by the o2 mutation and expressed during maize endosperm development
Characterization of novel proteins affected by the o2 mutation and expressed during maize endosperm development
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DOI：
10.1007/s004380050657
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发表时间：
1998-02-01
期刊：
影响因子：
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通讯作者：
Le Guilloux, M
中科院分区：
文献类型：
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作者：
Damerval, C;Le Guilloux, M
通过双向电泳（2-D PAGE）在7对不同的近等基因玉米品系中研究了o2突变对籽粒发育过程中蛋白质表达的影响。目的是确定一组蛋白质，这些蛋白质在突变体中始终受到影响，并且可能是转录激活因子O2直接或间接靶向的新基因的产物。发现36种多肽的丰度在七种背景中被修饰。17种多肽在野生型中的含量比突变体中的含量更高，其中大多数在早期就受到影响。其余的多肽在突变体中的表达水平高于野生型，并且通常在发育后期受到影响，这表明它们可能是O2的间接靶点的产物。已知的直接靶基因如玉米醇溶蛋白、B-32蛋白和丙酮酸正磷酸二激酶的产物包括在第一组多肽中。对13种多肽进行了15个氨基酸的内部延伸的微测序，发现其中9种多肽与数据库中存储的序列具有同源性。属于各种代谢途径的酶被初步鉴定，其中大部分以前不知道会受到o2突变的影响。这些结果证实了O2基因可以作为一个连接调节基因的不同途径的谷物代谢。
The effect of the o2 mutation on protein expression during grain development was examined by two-dimensional electrophoresis (2-D PAGE) in seven different pairs of near-isogenic maize lines. The aim was to identify a set of proteins that are consistently affected in mutants, and which could be the products of new genes that are direct or indirect targets of the transcriptional activator O2. The abundance of 36 polypeptides was found to be modified in the seven backgrounds. Seventeen polypeptides were present in greater amounts in wild types than in mutants, and most of these were affected early. The remaining polypeptides were expressed at higher levels in mutants than in the wild types and were generally affected later in development, suggesting that they might be products of indirect targets of O2. Products of known direct target genes such as zeins, b-32 protein and a pyruvate orthophospate dikinase were included in the first set of polypeptides. Microsequencing of internal stretches of 15 amino acids was performed for thirteen polypeptides and homologies with sequences stored in databases were found for nine of them. Enzymes belonging to various metabolic pathways were tentatively identified, most of which were not previously known to be affected by the o2 mutation. These results confirm that the O2 gene could act as a connecting regulatory gene for different pathways of grain metabolism.