Highly Fluorescent dye-nanoclay Hybrid Materials Made from Different Dye Classes
Highly Fluorescent dye-nanoclay Hybrid Materials Made from Different Dye Classes
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DOI：
10.1021/acs.langmuir.5b04297
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发表时间：
2016-04-12
期刊：
影响因子：
3.9
通讯作者：
Resch-Genger, Ute
中科院分区：
文献类型：
--
作者：
Grabolle, Markus;Starke, Marian;Resch-Genger, Ute
像laponite这样的纳米粘土，在商业上以非常适中的价格大量可用，为疏水染料提供了一种简便的增溶策略，而不需要化学功能化，并且可以作为大量染料分子的载体。这不需要活性染料，由于每个拉脱土圆盘上有大量的染料分子，因此放大了来自单个发射器的荧光信号，并且使疏水发射器适用于水环境。为了合理设计明亮染料负载纳米粘土作为生物分析和材料科学领域的一类新型荧光报告材料，以及染料结构性质关系的鉴定，我们筛选了一系列不同染料类别、电荷和所涉及的光学转变特征的商品荧光染料，研究了不同负载染料浓度下粘土吸附对其光学性质的影响。基于52105和Lumogen F Yellow 083的染料负载密度依赖性吸收和荧光测量，我们可以确定两种有前途的染料-纳米粘土杂化材料，纳米粘土吸附染料的荧光量子产率至少为0.20，即使在每个laponite血小板高达50个染料分子的高染料负载密度下，染料自猝灭率也很低。
Nanoclays like laponites, which are commercially avaible in large quantities for a very moderate price, provide a facile solubilization strategy for hydrophobic dyes without the need for chemical functionalization and can act as a carrier for a high number of dye molecules. This does not require reactive dyes, amplifies fluorescence signals from individual emitters due to the high number of dyes molecules per laponite disk, and renders hydrophobic emitters applicable in aqueous environments. Aiming at the rational design of bright dye-loaded nanoclays as a new class of fluorescent reporters for bioanalysis and material sciences and the identification of dye structure property relationships, we screened a series of commercial fluorescent dyes, differing in dye class, charge, and character of the optical transitions involved, and studied the changes of their optical properties caused by clay adsorption at different dye loading concentrations. Upon the basis of our dye loading density-dependent absorption and fluorescence measurements with 52105 and Lumogen F Yellow 083, we could identify two promising dye-nanoclay hybrid materials that reveal high fluorescence quantum yields of the nanoclay-adsorbed dyes of at least 0.20 and low dye self-quenching even at high dye-loading densities of up to 50 dye molecules per laponite platelet.