EXPRESSION OF MESSENGER-RNA ENCODING IFN-ALPHA IN MACROPHAGES STIMULATED WITH LACTOBACILLUS-GASSERI
EXPRESSION OF MESSENGER-RNA ENCODING IFN-ALPHA IN MACROPHAGES STIMULATED WITH LACTOBACILLUS-GASSERI
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DOI：
10.1016/0378-1097(94)90490-1
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发表时间：
1994-07-15
影响因子：
2.1
通讯作者：
YAMAGUCHI, T
中科院分区：
文献类型：
--
作者：
KITAZAWA, H;TOMIOKA, Y;YAMAGUCHI, T
在小鼠巨噬细胞培养中，研究了乳乳酸菌干酪乳杆菌诱导干扰素（IFN）的能力。部分菌株对IFN α有较强的诱导作用，在100 μ g ml（-1）的浓度下，IFN α的滴度最高。用L. gasseri DSM20243(T)刺激小鼠脾-巨噬细胞（SP-M phi）和Peyer's贴片贴壁细胞，检测IFN α编码mRNA的表达。添加放线菌素D和环己亚胺后，SP-M - phi培养物在0.5 h内合成mRNA，在3 ~ 6 h内产生IFN α。研究结果支持含有乳杆菌的乳制品可以是“生理功能食品”的观点。
The ability of Lactobacillus gasseri, a dairy lactic acid bacterium, to induce interferon (IFN) was investigated in murine macrophage cultures. IFN alpha was substantially induced by some strains of L. gasseri and the titers were the highest at a concentration of 100 mu g ml(-1) of L. gasseri DSM20243(T). The expression of mRNA encoding IFN alpha was detected in spleen-macrophages (SP-M phi) and Peyer's patch-adherent cells stimulated with L. gasseri DSM20243(T). Actinomycin D and cycloheximide added to SP-M phi cultures showed that the mRNA was synthesized by 0.5 h, and that IFN alpha was produced within 3 to 6 h after the stimulation with L. gasseri DSM20243(T). The results support the notion that dairy products containing L. gasseri can be 'physiologically functional foods'.