An Smc3 acetylation cycle is essential for establishment of sister chromatid cohesion.
An Smc3 acetylation cycle is essential for establishment of sister chromatid cohesion.
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DOI：
10.1016/j.molcel.2010.08.008
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发表时间：
2010-09-10
期刊：
影响因子：
16
通讯作者：
Nasmyth K
中科院分区：
文献类型：
--
作者：
Beckouët F;Hu B;Roig MB;Sutani T;Komata M;Uluocak P;Katis VL;Shirahige K;Nasmyth K
姐妹染色单体的凝聚被认为涉及由凝聚素亚基Smc1，Smc3和Scc1组成的三环对姐妹DNA的捕获。S期内聚性的建立依赖于Eco1乙酰转移酶对Smc 3的核苷酸结合结构域（NBD）的乙酰化。由于Scc1被分离酶切割，在后期开始时被破坏。在酵母中，Smc 3乙酰化在后期被Hos 1脱乙酰酶逆转，作为Scc 1裂解的结果。Smc3分子保持乙酰化后，有丝分裂由于Hos1失活不能产生凝聚力在随后的S期，这意味着凝聚力的建立依赖于从头乙酰化DNA复制过程中。通过诱导Smc3去乙酰化在postreplicative细胞由于Hos1过表达，我们提供的证据表明，Smc3乙酰化有助于维持姐妹染色单体凝聚力。因此，Smc 3 NBD乙酰化的循环是真核细胞中染色体周期的重要方面。
Sister chromatid cohesion is thought to involve entrapment of sister DNAs by a tripartite ring composed of the cohesin subunits Smc1, Smc3, and Scc1. Establishment of cohesion during S phase depends on acetylation of Smc3’s nucleotide-binding domain (NBD) by the Eco1 acetyl transferase. It is destroyed at the onset of anaphase due to Scc1 cleavage by separase. In yeast, Smc3 acetylation is reversed at anaphase by the Hos1 deacetylase as a consequence of Scc1 cleavage. Smc3 molecules that remain acetylated after mitosis due to Hos1 inactivation cannot generate cohesion during the subsequent S phase, implying that cohesion establishment depends on de novo acetylation during DNA replication. By inducing Smc3 deacetylation in postreplicative cells due to Hos1 overexpression, we provide evidence that Smc3 acetylation contributes to the maintenance of sister chromatid cohesion. A cycle of Smc3 NBD acetylation is therefore an essential aspect of the chromosome cycle in eukaryotic cells.