A new role for CsrA: promotion of complex formation between an sRNA and its mRNA target in Bacillus subtilis
A new role for CsrA: promotion of complex formation between an sRNA and its mRNA target in Bacillus subtilis
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DOI：
10.1080/15476286.2019.1605811
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发表时间：
2019-07-03
期刊：
影响因子：
4.1
通讯作者：
Brantl, Sabine
中科院分区：
文献类型：
--
作者：
Mueller, Peter;Gimpel, Matthias;Brantl, Sabine
CsrA是一种广泛保守的、丰富的小RNA结合蛋白，已在大肠杆菌中发现。大肠杆菌和其他革兰氏阴性细菌，其中它参与碳代谢、生物膜形成和毒力的调节。CsrA结合靶mRNA的SD序列周围的单链GGA基序，在那里它抑制或激活翻译或影响RNA加工。含有13-22个GGA基序的小RNA如CsrB或Csrc可以螯合CsrA，从而消除CsrA对其靶mRNA的作用。在B。在枯草芽孢杆菌中，CsrA迄今为止仅被发现调节一个靶点，hag mRNA，并且被蛋白质（FliW）而不是sRNA螯合。在这里，我们采用体外和体内相结合的方法来研究CsrA对来自B的小调节RNA SR 1的影响。枯草芽孢杆菌，其主要靶标ahrC mRNA及其下游靶标rocABC和rocDEF操纵子。我们证明，CsrA可以促进SR 1和ahrC mRNA之间的碱基配对相互作用，迄今为止只发现Hfq或ProQ的功能。缩写：aa，氨基酸; bp，碱基对; nt，核苷酸; PAA，聚丙烯酰胺; SD，Shine Dalgarno。
CsrA is a widely conserved, abundant small RNA binding protein that has been found in E. coli and other Gram-negative bacteria where it is involved in the regulation of carbon metabolism, biofilm formation and virulence. CsrA binds to single-stranded GGA motifs around the SD sequence of target mRNAs where it inhibits or activates translation or influences RNA processing. Small RNAs like CsrB or CsrC containing 13-22 GGA motifs can sequester CsrA, thereby abrogating the effect of CsrA on its target mRNAs. In B. subtilis, CsrA has so far only been found to regulate one target, hag mRNA and to be sequestered by a protein (FliW) and not by an sRNA. Here, we employ a combination of in vitro and in vivo methods to investigate the effect of CsrA on the small regulatory RNA SR1 from B. subtilis, its primary target ahrC mRNA and its downstream targets, the rocABC and rocDEF operons. We demonstrate that CsrA can promote the base-pairing interactions between SR1 and ahrC mRNA, a function that has so far only been found for Hfq or ProQ.Abbreviations: aa, amino acid; bp, basepair; nt, nucleotide; PAA, polyacrylamide; SD, Shine Dalgarno.