Comparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection
Comparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection
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DOI：
10.2807/1560-7917.es.2015.20.41.30042
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发表时间：
2015-10-15
期刊：
影响因子：
19
通讯作者：
Oh, M.
中科院分区：
文献类型：
--
作者：
Park, S. W.;Perera, R. A. P. M.;Oh, M.
使用症状发作后2 - 46天收集的17例MERS患者的95份血清，比较了空斑减少中和试验（PRNT）、微量中和试验（MN）、中东呼吸综合征（MERS）-刺突假颗粒中和试验（ppNT）和MERS S1-酶联免疫吸附试验（ELISA）抗体滴度。中和试验相互之间的相关性良好，与S1 ELISA的相关性中等。此外，为了比较遗传多样性MERS-CoV分支的抗原相似性，对在疾病过程中两个时间段采样的两名患者的四份血清的反应进行了90% PRNT测试。遗传多样的MERS CoV进化枝是抗原同源的。
Plaque reduction neutralisation tests (PRNT), micro-neutralisation (MN), Middle East respiratory syndrome (MERS)-spike pseudoparticle neutralisation (ppNT) and MERS S1-enzyme-linked immunosorbent assay (ELISA) antibody titres were compared using 95 sera from 17 patients with MERS, collected two to 46 days after symptom onset. Neutralisation tests correlated well with each other and moderately well with S1 ELISA. Moreover to compare antigenic similarity of genetically diverse MERS-CoV clades, the response of four sera from two patients sampled at two time periods during the course of illness were tested by 90% PRNT. Genetically diverse MERS-CoV clades were anti-genically homogenous.