IMMUNOGLOBULIN M-IMMUNOSORBENT AGGLUTINATION ASSAY FOR DIAGNOSIS OF INFECTIOUS-DISEASES - DIAGNOSIS OF ACUTE CONGENITAL AND ACQUIRED TOXOPLASMA INFECTIONS
IMMUNOGLOBULIN M-IMMUNOSORBENT AGGLUTINATION ASSAY FOR DIAGNOSIS OF INFECTIOUS-DISEASES - DIAGNOSIS OF ACUTE CONGENITAL AND ACQUIRED TOXOPLASMA INFECTIONS
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DOI：
10.1128/jcm.14.5.486-491.1981
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发表时间：
1981-01-01
影响因子：
9.4
通讯作者：
REMINGTON, JS
中科院分区：
文献类型：
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作者：
DESMONTS, G;NAOT, Y;REMINGTON, JS
IgM免疫吸附凝集试验（IgM-ISAGA）在Sabin-Feldman染料试验阴性个体的所有血清、慢性弓形虫感染个体的血清和未感染婴儿的脐带血样本中均为阴性。与此相反，所有的血清，从个人获得的最近的历史急性弓形虫感染和先天性弓形虫感染的婴儿，并在染料试验和IgM-间接荧光抗体（IgM-IFA）试验阳性的IgM-ISAGA阳性。尽管从最近获得的弓形虫感染的个体获得，但在IgM-IFA试验中呈阴性的31份血清中共有21份（67.7%），并且在IgM-IFA试验中呈阴性且从先天性弓形虫感染的婴儿获得的11份血清中有8份（72.7%）在IgM-ISAGA中呈阳性。类风湿因子、抗核抗体或两者的存在不会导致IgM-ISAGA假阳性结果，但会导致IgM-IFA测试假阳性结果。IgM-ISAGA比IgM-IFA更敏感、特异。弓形虫感染的诊断和急性先天性和获得性弓形虫感染的诊断。
An IgM immunosorbent agglutination assay (IgM-ISAGA) was negative in all sera from individuals negative in the Sabin-Feldman dye test, in sera from individuals with chronic Toxoplasma infection, and in cord blood samples from uninfected infants. In contrast, all sera that were obtained from individuals with a recent history of acute Toxoplasma infection and from infants with congenital Toxoplasma infection and that were positive in both the dye test and the IgM-indirect fluorescent-antibody (IgM-IFA) test were positive in IgM-ISAGA. A total of 21 (67.7%) of 31 sera that were negative in the IgM-IFA test, despite being obtained from individuals with recently acquired Toxoplasma infection, and 8 (72.7%) of 11 sera that were negative in the IgM-IFA test and obtained from infants with congenital Toxoplasma infection were positive in IgM-ISAGA. The presence of rheumatoid factor, antinuclear antibodies or both did not cause false-positive results in the IgM-ISAGA but did so in the IgM-IFA test. IgM-ISAGA is both more sensitive and more specific than the IgM-IFA test for detection of IgM antibodies to T. gondii and for the diagnosis of acute congenital and acquired Toxoplasma infections.