The human VASA gene is specifically expressed in the germ cell lineage
The human VASA gene is specifically expressed in the germ cell lineage
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DOI：
10.1073/pnas.160274797
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发表时间：
2000-08-15
影响因子：
11.1
通讯作者：
Crum, CP
中科院分区：
文献类型：
--
作者：
Castrillon, DH;Quade, BJ;Crum, CP
为了了解人类生殖细胞谱系的起源和功能，并鉴定生殖细胞特异性标记，我们分离了果蝇基因花瓶的人类同源基因，通过辐射杂交将该基因定位到人类染色体5q(靠近着丝粒)。我们通过对胎儿和成人组织的Northern分析表明，人类VASA基因的表达仅限于卵巢和睾丸，在体细胞组织中检测不到。我们产生了与福尔马林固定的石蜡包埋组织中的Vasa蛋白结合的多克隆抗体，并表征了不同发育阶段的人类生殖细胞中Vasa蛋白的表达。Vasa蛋白是细胞质的，在性腺脊区的迁徙原始生殖细胞中表达。VASA蛋白在胎儿和成人性腺生殖细胞中均有表达，在精母细胞和成熟卵母细胞中含量最高，因此我们所分离的基因是生殖细胞高度特异的标志物，可用于研究人类生殖细胞的决定和功能。
To understand the origins and function of the human germ cell lineage and to identify germ cell-specific markers we have isolated a human ortholog of the Drosophila gene vase, The gene was mapped to human chromosome 5q (near the centromere) by radiation hybrid mapping. We show by Northern analysis of fetal and adult tissues that expression of the human VASA gene is restricted to the ovary and testis and is undetectable in somatic tissues. We generated polyclonal antibodies that bind to the VASA protein in formalin-fixed, paraffin-embedded tissue and characterized VASA protein expression in human germ cells at various stages of development. The VASA protein is cytoplasmic and expressed in migratory primordial germ cells in the region of the gonadal ridge. VASA protein is present in fetal and adult gonadal germ cells in both males and females and is most abundant in spermatocytes and mature oocytes, The gene we have isolated is thus a highly specific marker of germ cells and should be useful for studies of human germ cell determination and function.