Virus-Mediated Expression of DREADDs for In Vivo Metabolic Studies.
Virus-Mediated Expression of DREADDs for In Vivo Metabolic Studies.
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DOI：
10.1007/978-1-4939-2914-6_14
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Wess, Jurgen
中科院分区：
文献类型：
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作者：
Rossi, Mario;Cui, Zhenzhong;Nakajima, Ken-ichiro;Hu, Jianxin;Zhu, Lu;Wess, Jurgen
在过去的几年中，HOC敏感的设计师G蛋白偶联受体（GPCR）被称为DREADD（设计师受体专门由设计师药物激活）已经成为研究GPCR生理学的强大新工具。在这一章中，我们提出的协议采用腺相关病毒（AAVs）表达Gq耦合DREADD（Dq）在两个代谢重要的细胞类型，下丘脑和肝细胞的AgRP神经元。我们还提供了在体内施用CNO后处理Dq突变小鼠的代谢分析的实例。本章中描述的方法可以应用于DREADD家族的其他成员，当然也可以应用于不同的细胞类型。DREADD技术的使用很可能会识别出生理学上重要的信号通路，这些通路可以用于治疗目的。
During the past few years, CNO-sensitive designer G protein-coupled receptors (GPCRs) known as DREADDs (designer receptors exclusively activated by designer drugs) have emerged as powerful new tools for the study of GPCR physiology. In this chapter, we present protocols employing adeno-associated viruses (AAVs) to express a Gq-coupled DREADD (Dq) in two metabolically important cell types, AgRP neurons of the hypothalamus and hepatocytes of the liver. We also provide examples dealing with the metabolic analysis of the Dq mutant mice after administration of CNO in vivo. The approaches described in this chapter can be applied to other members of the DREADD family and, of course, different cell types. It is likely that the use of DREADD technology will identify physiologically important signaling pathways that can be targeted for therapeutic purposes.